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Bioreactors

Animal cell BioReactor VMF Reactor

Vertical reciprocating motion type mixer for cell culture

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The VMF reactor is a vertical reciprocating motion type next-generation reciprocating mixer for cell culture that combines together excellent blending performance and gentle mixing. Unlike the general rotating type, severe shear stress control is possible. Moreover, a “completely enclosed structure” that does not require a complex rotating-axis seal mechanism is implemented. There is no threat of contamination or leakage, and it has a high level of sterility and cleanliness.
With the help of the dedicated controller “S-BOX × 10α”, changes in various parameters including PI control can be easily performed. Through customization, up to eight machines in series are supported.

Mixing impeller used exclusively for vertical reciprocating motion

Control of the shearing action and a good mixing action are both established, and an environment that is best suited for cell culture is created!!

■Comparison of shear stress

■Culture example

Comparison of cell culture by CHO cells

Here we show the cell viability in the later stage of the culture under the batch cell culture condition for the turbine type (flat turbine) and wide paddle type (elephant ear) impellers used in the conventional rotating type BioReactor. It can be confirmed that the MRF reactor maintains a higher cell viability than the other conventional impellers in the later stage of the culture. A characteristic of the MR210Bio that has a low shearing action and high mixing homogeneity performance is the realization of scale-up to a large-size reactor through the combined use of numerical fluid calculation.

■VMF Reactor Specifications
Item		Specifications
Product name		VMF Reactor
Model		VMF-500	VMF-1500	VMF-3000	VMF-10000
Temperature control type		Band heater method (PID control) with excessive temperature rise prevention function（MAX 80℃）
Linear-motion transmission method		Linear shaft drive non-sealing type
Ventilation type		Sintering metal type*Optional
Performance	Temperature regulation range	Room temperature+5～20℃ (normally set to 37℃)
	Temperature regulation accuracy	±0.3℃(37℃)
	Max. up/down motion amplitude	40ｍｍ
	Max. impeller speed	300mm/s
Function	Temperature setting	Digital setting
Function	Up/down motion setting	Touch panel input
Configuration	Band heater	150W
	Mixing motor	Max. output　800W
	Mixing impellers	Standard VM200	Standard VM100 + VM200
Standard	Culture tank dimensions	Inner diameter90 ×200Depth [mm]	Inner diameter110 ×169Depth [mm]	Inner diameter140 ×203Depth [mm]	Inner diameter200 ×360sDepth [mm]
Standard	Culture tank total capacity	0.5L	1.5L	3L	10L
Operating ambient temperature range		10~35℃
Outer dimensions		300W×485D×890H[mm] (When a fall prevention fitting has been attached、360W)
Weight		Approx. 28kg	Approx. 28kg	Approx. 30kg	Approx. 34kg
Rated power supply		AC-100V, 50/60Hz

* The performance is the value at a room temperature of 20℃, rated power supply voltage 100 VAC 50 Hz, during no load.

■S-BOX×10αSpecifications
Item	Specifications
Name	Culture controller
Model	S-BOX×10α
Display range / accuracy	pH	0.00～14.00 [pH]	Display accuracy：F.S±0.5%
	DO(Dissolved oxygen)	0.00～10.00 [mg/L]
	FL(O₂ flow rate)	0.00～20.00 [mL/min]
Control setting range	pH	0.00～14.00 [pH]
	DO(Dissolved oxygen)	0.00～10.00 [mg/L]
	FL(O₂ flow rate)	0.00～20.00 [mL/min]
Control output	pH	ON/OFF control
	DO(Dissolved oxygen)	Flow rate addition/subtraction step control
	FL(O₂ flow rate)	PI control (slope set method: Time, %)
Record output	pH	Data logger included DC 0～5 [V]	Output accuracy：F.S±0.5%
	DO(Dissolved oxygen)
	FL(O₂ flow rate)
Power supply	AC 100 V, 50/60Hz
Case material	SUS304 （No coating） Indoor type, non-drip proof, non-explosion proof specification
Installation method	Indoor tabletop type
Outer dimensions	W260×Ｈ350×D300 [mm]
Ambient environment conditions	Temperature	5~45 ℃
Ambient environment conditions	Humidity	20~85 %（No dew formation）
Utility	Power supply	100 VAC electrical outlet × 2 (for controller unit and recorder)
	O₂	Flow rate 20 ml/min or less Supply pressure 0.3 MPa Connection port Φ6 tube one-touch joint
	CO₂	Flow rate 50 ml/min or less Supply pressure 0.2 MPa Connection port Φ6 tube one-touch joint
	AIR	Flow rate 150 ml/min or less Supply pressure 0.2 MPa Connection port Φ6 tube one-touch joint
	* O₂, CO₂, and AIR must all be dry gases that do not contain corrosive components, dust, and oil mist