Products & Services

Products & Services

Bioreactors

Animal cell BioReactor VMF Reactor

Vertical reciprocating motion type mixer for cell culture

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VMF Reactor
The VMF Reactor is a vertical reciprocating motion type next-generation reciprocating mixer for cell culture that combines together excellent blending performance and gentle mixing. Unlike the general rotating type, severe shear stress control is possible. Moreover, a “completely enclosed structure” that does not require a complex rotating-axis seal mechanism is implemented. There is no threat of contamination or leakage, and it has a high level of sterility and cleanliness.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed. Through customization, up to eight machines in series are supported.

Mixing impeller used exclusively for vertical reciprocating motion

Mixing impeller used exclusively for vertical reciprocating motion

VM100

VM200

Control of the shearing action and a good mixing action are both established, and an environment that is best suited for cell culture is created!!

Comparison of shear stress

CFD Simulation Analysis Result

Culture example

Culture example

Comparison of cell culture by CHO cells

The VMF reactor provides cells with the optimal physical environment in the culture tank, creating a suitable tank environment for cell cultivation compared to rotational BioReactors, maintaining a high rate of living cells compared to MRF reactors.
Although this superior effect is weakened for cells that are resistant to shear damage, this product offers superior performance for cell culture that relies on shear damage. This results in increased productivity.


Item Specifications
Name VMF Reactor
Model VMF-500 VMF-1500 VMF-3000 VMF-10000
Temperature control method Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission Linear shaft drive non-sealing type
Gas supply device Sintered metal type *Optional
Performance Temp. control range Room temperature + 5 to 20℃ (normally set to 37℃)
Temp. accuracy ±0.3[℃](37[℃])
Max. up/down motion amplitude 40[mm]
Max. impeller speed 300[mm/s]
Function Temperature setting Digital setting, data output 1 - 5 [V]
Up/down motion setting Touch panel input, data output 1 - 10 [V]
Configuration Band heater 60 [W] 105 [W] 160 [W] 480 [W]
Mixer motor Max. power 800 [W]
Mixing impellers [Standard] VM200 [Standard] VM100+VM200
Culture tank Dimensions I.D. 90 x Depth 200 [mm] I.D. 110 x Depth 169 [mm] I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]
Total capacity 0.5[L] 1.5[L] 3[L] 10[L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm]
Weight approx. 28[kg] approx. 28[kg] approx. 30[kg] approx. 34[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×10αII
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: time, %)
FL (O2flow rate) PI control (slope set method: time, %)
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
*Load factor can be put out. (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL (O2flow rate)
Mixing frequency
Temperature With data logger
DC 1 - 5 [V]
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×H530×D400 [mm] ・ approx. 15[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
N2 Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.