Products & Services

Products & Services


Animal cell BioReactor VMF Reactor

Vertical reciprocating motion type mixer for cell culture

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VMF Reactor
The VMF Reactor is a vertical reciprocating motion type next-generation reciprocating mixer for cell culture that combines together excellent blending performance and gentle mixing. Unlike the general rotating type, severe shear stress control is possible. Moreover, a “completely enclosed structure” that does not require a complex rotating-axis seal mechanism is implemented. There is no threat of contamination or leakage, and it has a high level of sterility and cleanliness.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed. Through customization, up to eight machines in series are supported.

Mixing impeller used exclusively for vertical reciprocating motion

Mixing impeller used exclusively for vertical reciprocating motion



Control of the shearing action and a good mixing action are both established, and an environment that is best suited for cell culture is created!!

Comparison of shear stress

CFD Simulation Analysis Result

Culture example

Culture example

Comparison of cell culture by CHO cells

The VMF reactor provides cells with the optimal physical environment in the culture tank, creating a suitable tank environment for cell cultivation compared to rotational BioReactors, maintaining a high rate of living cells compared to MRF reactors.
Although this superior effect is weakened for cells that are resistant to shear damage, this product offers superior performance for cell culture that relies on shear damage. This results in increased productivity.

Item Specifications
Name VMF Reactor
Model VMF-05 VMF-1 VMF-3 VMF-10
Temp. control device Band heater (PID control) with over-temperature protection function (Max. 80 [℃])
Power transmission Linear shaft drive non-sealing type
Gas supply type Liquid surface gas absorption (option: sintered metal type)
Performance*1 Temp. control range Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
Temp. accuracy ±0.3 [℃](37 [℃])
Max. shaft stroke 40 [mm]
Max. shaft speed 300 [mm/s]
Function Temp. setting Touch panel input, data output DC1 - 5 [V]
Vertical motion setting Touch panel input, data output DC0 - 5 [V]
Configuration Power of band heater Max. 60 [W] Max. 105 [W] Max. 160 [W] Max. 480 [W]
Power of motor Max. output 800 [W]
Mixing impellers VM200 VM100+VM200*2
Culture tank Dimensions I.D. 90 x Depth 200 [mm] I.D. 110 x Depth 169 [mm] I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]
Culture operation volume 0.3 [L] 1.2 [L] 2.4 [L] 7 [L]
Usage condition 10 - 35 [℃]
Outer dimensions W360×D485×H905 [mm]
Weight Approx. 28 [kg] Approx. 28 [kg] Approx. 30 [kg] Approx. 34 [kg]
Power supply AC100 [V], 50/60 [Hz]
*1 The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.
*2 Depending on the Culture operation volume, the only mixing impeller may be only the VM200.

Item Specifications
Name Culture controller
Model S-BOX×10αII
Control pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump
Display range/accuracy pH (Hydrogen ion concentration) 0.00 - 14.00 [-] Display accuracy: ±0.5 [%] F.S.
DO (Dissolved oxygen) 0.00 - 20.00 [mg/L]
FL (O2flow rate) 0.00 - 20.00 [mL/min]
Setting range pH (Hydrogen ion concentration) 0.00 - 14.00 [-]
DO (Dissolved oxygen) 0.00 - 10.00 [mg/L]
FL (O2flow rate) 0.00 - 20.00 [mL/min]
Control type pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: TIME, %)
FL (O2flow rate) PI control (slope set method: TIME, %)
Data output pH (Hydrogen ion concentration) With data logger DC0 - 5 [V]
(option : Load factor can be outputted.)
Accuracy: ±0.5 [%] F.S.
DO (Dissolved oxygen)
FL (O2flow rate)
Mixing frequency
Temperature sensor DC1 - 5 [V]
MTA of outer surface SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×D400×H530 [mm] ・ Approx. 15 [kg]
Usage conditions Temperature 5 - 45 [℃] Humidity 20 - 85 [%] RH (No condensation)
Sensors Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company
(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd. )
Utilities Power supply AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
N2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [Mpa] connection port φ6 one touch tube fitting
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
*All of N2, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.