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Animal cell BioReactor VMF Reactor

Vertical reciprocating motion type mixer for cell culture

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The VMF Reactor is a vertical reciprocating motion type next-generation reciprocating mixer for cell culture that combines together excellent blending performance and gentle mixing. Unlike the general rotating type, severe shear stress control is possible. Moreover, a “completely enclosed structure” that does not require a complex rotating-axis seal mechanism is implemented. There is no threat of contamination or leakage, and it has a high level of sterility and cleanliness.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed. Through customization, up to eight machines in series are supported.

Mixing impeller used exclusively for vertical reciprocating motion

VM100

VM200

Control of the shearing action and a good mixing action are both established, and an environment that is best suited for cell culture is created!!

■Comparison of shear stress

■Culture example

Comparison of cell culture by CHO cells

The VMF reactor provides cells with the optimal physical environment in the culture tank, creating a suitable tank environment for cell cultivation compared to rotational BioReactors, maintaining a high rate of living cells compared to MRF reactors.
Although this superior effect is weakened for cells that are resistant to shear damage, this product offers superior performance for cell culture that relies on shear damage. This results in increased productivity.

▼　VMF Reactor Specifications（Click to open details）

Item		Specifications
Name		VMF Reactor
Model		VMF-500	VMF-1500	VMF-3000	VMF-10000
Temperature control method		Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission		Linear shaft drive non-sealing type
Gas supply device		Sintered metal type *Optional
Performance	Temp. control range	Room temperature + 5 to 20℃ (normally set to 37℃)
	Temp. accuracy	±0.3[℃]（37[℃]）
	Max. up/down motion amplitude	40[mm]
	Max. impeller speed	300[mm/s]
Function	Temperature setting	Digital setting, data output 1 - 5 [V]
Function	Up/down motion setting	Touch panel input, data output 1 - 10 [V]
Configuration	Band heater	60 [W]	105 [W]	160 [W]	480 [W]
	Mixer motor	Max. power 800 [W]
	Mixing impellers	[Standard] VM200	[Standard] VM100+VM200
Culture tank	Dimensions	I.D. 90 x Depth 200 [mm]	I.D. 110 x Depth 169 [mm]	I.D. 140 x Depth 203 [mm]	I.D. 200 x Depth 360 [mm]
Culture tank	Total capacity	0.5[L]	1.5[L]	3[L]	10[L]
Environment temp. range		10～35[℃]
Outer dimensions		W360×H905×D485 [mm]
Weight		approx. 28[kg]	approx. 28[kg]	approx. 30[kg]	approx. 34[kg]
Power supply		AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

▼　S-BOX×10αⅡ Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×10αII
Display range/accuracy	pH (Hydrogen ion concentration)		0.00～14.00 [pH]		Display accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)		0.00～20.00 [mg/L]
	FL (O₂flow rate)		0.00～20.00 [mL/min]
Setting range	pH (Hydrogen ion concentration)		0.00～14.00 [pH]
	DO (Dissolved oxygen)		0.00～10.00 [mg/L]
	FL (O₂flow rate)		0.00～20.00 [mL/min]
Control output	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: time, %)
	FL (O₂flow rate)		PI control (slope set method: time, %)
Data output	pH (Hydrogen ion concentration)		With data logger DC 0 - 5 [V] *Load factor can be put out. (optional)		Accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)
	FL (O₂flow rate)
	Mixing frequency
	Temperature		With data logger DC 1 - 5 [V]
Casing	SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×H530×D400 [mm]　・　approx. 15[kg]
Environment conditions	Temperature	5～45 [℃]	Humidity	20 - 85% RH (with no condensate)
Sensors	Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Sensors	Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities	Power supply	AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.