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CSS II continuous culture and perfusion system connectable to Satake BioReactor

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

CSS II continuous culture and perfusion system
Culture medium replacement inside the culture tank utilizes the electronic balance and feed pump attached to the controller (CSS II) to supply culture medium at the set time and weight and simultaneously discharge the culture medium.
The feed pump automatically calculates the optimal number of revolutions from the set time, pump flow rate value (select the tube diameter) and current weight value of the electronic balance, and operation continues until the specified time. Additionally, the balance weight is monitored every 0.1 seconds during control operations, and the number of pump revolutions automatically changes. Control automatically stops once the specified time is reached. The standard included pump is generally used at medicine manufacturing sites and in culture laboratories: Watson-Marlow 120U/DV analogue control variable speed pump, and electronic balance: A&D GX3002A included as a standard.

Introducing an example system

The continuous culture and perfusion system CSS II device flow and system flow are shown in the figure. Connecting the supply and discharge pump and electronic balance as shown in the figure enables automatic culture medium replacement.
Additionally, a single CSS II can be used for culture medium replacement in two culture tanks.

■system flow

system flow

※The supply and discharge of the system flow chart can be controlled up to 2 systems.

We also provide pump systems and perfusion systems tailored to customer needs.
Please feel free to contact us regarding specialized designs for actual production, such as a single balance/pump configuration, and liquid surface control to prevent adhesion during culturing and production while conducting perfusion.


Item Specifications
Name Double Medium Replacement Controller
Model CSSⅡ
Display range Supply balance weight W1 0.01 - 3200.00 [g]
*The specification can be customized in accordance with customer's requirement.
Discharge balance weight W2
Supply balance weight W3
Discharge balance weight W4
Setting range Supply pump P1 supply volume 0.0 - 3200.00 [g]
Display accuracy: ±0.5 [%] F.S.
*The specification can be customized in accordance with customer's requirement.　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　
Discharge pump P2 discharge volume
Supply pump P3 supply volume
Discharge pump P4 discharge volume
Culture medium supply / discharge time setting 1 [minute]　~　9999 [hours] 99 [minutes]
Control type Culture medium supply Continuous culture medium supply control by weight and fluid feed pump
Culture medium discharge
Record Data Balance weight W1, W2, W3, W4
Pump discharge volume P1, P2, P3, P4
MTA of outer surface SUS304 (no paint), Indoor type, non-waterproof, non-explosion proof specification
Installation Tabletop
Outer dimensions/weight W260×D400×H225 [mm] ・Approx. 10[kg]
Usage conditions Temperature 5 - 45 [℃] Humidity 20 - 85 [%] RH (No condensation)
Electronic balance/pump Electronic balance: A&D Company GX3002A x 2 (Option: GX-22001M)
Pump: Watson-Marlow 120U x 2
Utilities Power supply AC100 [V], 50/60 [Hz] electrical outlet 9 gang
(Main control unit, electronic balance x 4, fluid feed pump x 4)

Use with SATAKE perfusion systems

Low-temperature Incubator SLI-4090

Culture medium temperature meets temperature demands for 3 - 10℃

This incubator keeps the culture medium stable at a low temperature when used with TCS and CSSII perfusion systems.
There are two products in the lineup for use not only in labs but also in cell processing centers (CPC).
With an assumed culture medium temperature display function, this incubator enables more accurate temperature management than others.
A culture medium storage vessel equal to or smaller than the multipurpose Bag can be accommodated.
※A culture medium storage Bottle up to 2L vessel can be accommodated.

Low-temperature Incubator
  • ・Able to adjust culture medium temperature to any temperature between 3 - 10℃ ± 1.0℃ (ambient temperature 19℃)
  • ・Equipped with frost removal function (once every 8 hours approx.)
  • ・Equipped with automatic condensate evaporator function
  • ・CPC model equipped with low pressure loss HEPA filter
  • ・CPC model equipped with assumed culture medium temperature analog output function
  • ・Equipped with culture medium supply tube holder

Item Specifications
Name Low-temperature Incubator
Model SLI-4090-C
Environmental conditions Environment temp. range +18～22[℃] *1
Power supply AC100[V] 50/60[Hz] *2
Rated current 5[A]
Installation space Ensure space of at least 10cm around the main unit
Temperature control Internal temperature Setting range +3.0～10.0[℃] *3
Accuracy ±2.5[℃] (at center of inside) *4
Temperature display Digital display (1. internal temperature display, 2.② flask interior fluid assumed temperature display) *3, 5
Temperature correction Single point correction
Cooling type Refrigerator (freon gas R-134a filling volume 170 [g])
Mixing type Forced mixing by internal fan motor
Safety devices Electrical leakage/overcurrent breaker, fuse, anti-icing thermostat *6
Other functions ・Frost removal function (forced stop of refrigerator for approx. 15 mins once every 8 hours approx.)
・Automatic condensate evaporator function
・Hose holder (for O.D. φ6/installed at the bottom right side of interior access port)
・Left side surface of the unit has a φ36 hole accessing to the interior.
Internal dimensions W505×H461×D386[mm]
Outer dimensions W600×H643×D696 [mm]
Weight approx. 90[kg]
Standard accessories Power cable x 1 / fuse x 1 (in receptacle) / light plug x 1
*1: Must be no condensate in main unit. May not fulfill product specifications at temperatures other than the above environmental temperatures.
*2: Voltage fluctuation tolerance ±10 [%]
*3: At shipment, the internal setting temperature are adjusted so that the assumed temperature of the liquid in the flask is within +6.0±1.0 [°C] (@19.0 [°C]).
*4: In environment temp. 19 [℃]. This does not include temperature changes due to the frost removal function.
*5: The assumed temperature of the liquid in the flask is the temperature of the measuring rod located at the upper left rear inside the unit.
*6: Activates when an irregular low temperature is detected. Forces the cooler to stop when it activates (with automatic reactivation)

Item Specifications
Name Low-temperature Incubator
Model SLI-4090-B
Environmental conditions*1 Environment temp. range +18～22[℃] *2
Power supply AC100[V] 50/60[Hz] *3
Rated current 5[A]
Installation space Ensure space of at least 10cm around the main unit
Temperature control Internal temperature Setting range +3.0～10.0[℃] *4
Accuracy ±2.5 [℃] (at center of inside) *5
Temperature display Digital display (1. internal temperature display, 2. flask interior fluid assumed temperature display) *4, 6
Temperature correction Single point correction
Cooling type Refrigerator (freon gas R-134a filling volume 170 [g])
Mixing type Forced mixing by internal fan motor
Safety devices Electrical leakage/overcurrent breaker, fuse (built in), sensor cut off,
sample protection high/low temperature alarm (factory settings *7), safety device for built-in HEPA filter
(Circuit protector 1 [A], high temperature safety device 90 [℃])
Anti-icing thermostat *8
Output to external *9 Temperature Pt100 [Ω] sensor (-20 to +50 [℃]: 0 to 5[V])
Alarm High/low temp. alarm output (normal: open, error: close / AC250 [V] 1 [A] resistive load)
Other functions ・Frost removal function (forced stop of refrigerator for approx. 15 mins once every 8 hours approx.)
・Automatic condensate evaporator function
・Hose holder (for O.D. φ6/installed at the bottom right side of interior access port)
・Left side surface of the unit has a φ36 hole accessing to the interior.
HEPA filter ・Low pressure loss HEPA filter 400 × 400 × t50 [mm]
Air flow approx. 3.0[㎥/min]
Manostar gauge Pressure range 0 - 300 [Pa]
Internal dimensions W505×H461×D386[mm]
Outer dimensions W626×H830～900×D696 [mm]
Weight approx. 110[kg]
Standard accessories Power cable x 1 / fuse x 1 (in receptacle) / light plug x 1 / temperature sensor test report x1
*1: Cleanliness class of the installation environment is ISO class 7 or higher.
*2: Must be no condensate in main unit. May not fulfill product specifications at temperatures other than the above environmental temperatures.
*3: Voltage fluctuation tolerance up to ±10 [%] When decontaminating the room, turn off the breaker of the main unit and put the cover to the refrigerator air inlet.
*4: At shipment, the internal setting temperature are adjusted so that the assumed temperature of the liquid in the flask is within +6.0±1.0 [°C] (@19.0 [°C]).
*5: In environment temp. 19 [℃]. This does not include temperature changes due to the frost removal function.
*6: The assumed temperature of the liquid in the flask is the temperature of the measuring rod located at the upper left rear inside the unit.
*7: Sample material protect alarm setting: Low temperature -5 [℃]/high temperature 20 [℃]
*8: Activates when an irregular low temperature is detected. Forces the cooler to stop when it activates (with automatic reactivation)
*9: 2[m] cable from main unit, cable terminal: Y terminal
・High/low temperature alarm (normal: open, error: close (line color: black/green))
・Temperature data (0 - 5 [V], line color: red/white)

Optional items that can be connected to Satake BioReactors

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

■Aeration unit

Aeration unit
This unit is equipped with a compressor for easy air supply to a compact culture tank.

【Feature】
・Compact size for easy installation even in narrow spaces.
・Lightweight for air tank-style installations, eliminating the
　need to find installation locations.

Item Specifications
Name Aeration Unit
Model SAU-3505 SAU-3525 SAU8050 SAU80100
Performance Flow meter* 50～500[NmL/min] 0.2～2.5[NL/min] 0.5～5[NL/min] 1.0～10[NL/min]
Compressor Rated pressure 6.86×104[Pa] (0.7[kgf･cm2]) 9.81×104[Pa] (1.0[kgf･cm2])
Rated flow rate 3.5 [L/min] 8 [L/min]
Max. pressure 9.81×104[Pa] (1.0[kgf･cm2]) 1.47×105[Pa] (1.5[kgf･cm2])
Configuration Flow rate adjustment Constant flow valve for secondary pressure fluctuation
Air outlet O.D. φ8[mm]
others Outer dimensions W136×H236×D306 [mm] W136×H301×D306 [mm]
Power consumption 0.25[A]　25[VA] 0.6[A]　60[VA]
Power supply AC100[V]　50/60[Hz]
Weight approx. 6[kg] approx. 8[kg]
Remarks Needle valve (air relief valve) built in
*[NmL/min] or [NL/min] indicates the flow rate converted for 0 [℃] and 1 [atm] (101.3[kPa])

■Single-use pressure control system

Single-use pressure control system
This pressure controller enables culture tank pressure control at a constant level.

【Feature】
・It can be connected to a glass culture tank or single-use
　culture tank and the digital pressure switch regulates the
　electromagnetic valve for maintaining the culture tank
　pressure at a constant level.
・Fine tuning the opening of the manual bypass valve controls
　hunting, enabling mild control.

Item Specifications
Name In-tank pressure controller
Model Si-P1
Display range Pressure: -10.00 - 10.00 [kPa], Display accuracy: ± 2% (F.S. ± 1 digit)
Setting range Pressure: -10.50 - 10.50 [kPa]
Control type ON/OFF control
Output Analog signal: 1 - 5 [V]
Casing SUS304 (no paint), indoor type, non-drip proof, non-explosion proof specification
Installation Tabletop
Outer dimensions W125 × H100 × D225 [mm]
Environment conditions Pressure resist: 50 [kPa], temperature: 5 - 45 [℃], humidity: 20 - 85% RH (with no condensate)
Utilities AC100 - 240 [V] 50/60 [Hz] 0.5 [A] electrical outlet

■Cooling water circulator - Satake Cool Ace SCA-32

Satake Cool Ace SCA-32
The Cool Ace is a cooling water circulator that uses a refrigeration unit to chill the liquid in the included reservoir for external circulation, cooling each component that generates heat, such as the evaporator (1 L), culture tank, reaction tank, and various analytical devices and other equipment.

【Feature】
・It is a compact, tabletop type that can be installed even in
　narrow spaces.
・In addition to cooling the heat producing components of the
　BioReactor, it can be used for a variety of other purposes,
　such as analytical equipment.
・Temperature control is achieved with a simple panel.
・Temperature settings range from -20 to 30 ℃ for versatile
　applications.
・The circulation nozzle can be moved in 360°, reducing the
　load on tubes.

【Use Case】
Culture tank, cooling tube attached to reactor tank, culture tank temperature controller, viscosity gauge, etc.

Item Specifications
Name Coolant Circulator Cool Ace
Model SCA-32
Circulation type Closed system directional circulation
Performance Temperature control range -20～30 [℃]
Temperature accuracy Setting -1 ～ -20 [℃]: ±2 [℃], setting 0 ～ 20 [℃]) ±1 [℃]
Cooling -10[℃]：310 [W]　10[℃]：450 [W]　0[℃]：350 [W]
Circulation Max. lifting height 4.2 [m] ±0.5 / 5.6 [m] ±0.5 (50/60 [Hz])
Max. flow rate 9 [L/min] / 10 [L/min] (50/60 [Hz])
Function Temperature control type Refrigerator ON/OFF control
Temperature setting/display Sheet key digital setting configuration, switching digital display of measured temperature or set temperature (resolution: 1 [℃])
Safety functions Electrical leakage/overcurrent breaker, refrigerator overload relay, self-diagnostic functions (refrigerator error, sensor error, watchdog timer),
refrigerator protection timer, circulation pump impedance protection
Optional functions Flow control valve, metal nozzle, cool keeping hose set, trolley, product fixing parts,
coolant communication cable, communication cable (for linking with NVC-3000)
Configuration Temperature control Electronic digital setting configuration and digital display
Temperature sensor Pt sensor
Refrigerator/refrigerant Air-cooled, 450 [W], HFC, R-404A
Water tank Total capacity 3.2 [L] approx. Actual capacity 2.7 [L] approx. Material SUS304
Cooling coil SUS316L
Circulation nozzle O.D. 10 [mm] × I.D. 6.5 [mm]
Tank inner dimensions W130×H115×D230 [mm]
Environment temp. range 5～35 [℃]
Outer dimensions W205×H545×D405 [mm]
Weight approx. 28 [kg]
Power consumption 8 [A]、 800 [VA]
Power supply AC100 [V] 、50/60 [Hz]

Cultivation, cleaning, and concentration in a single line!
Cell concentration cleaning system

Cell concentration cleaning system

As an improvement on the cell culture system, we sell a connected, completely closed system that consists of our reactor and attached Kaneka Corporation cell concentration cleaning system. This system can be customized upon request.
From culture to cell washing and concentration, this product enables consistent processing in a closed system, streamlining the work process. A pump is built into the cell wash concentrator body, making preparations unnecessary, enabling simple installation by following the pictures and colors on the accessories and body.

Disposable Kit for Cell Concentration Washer

Disposable Kit for Cell Concentration Washer

●Sterilization method
Filter: γ sterilization
Circuit: EOG sterilization

●Safety
Passed the test specified by ISO10993(Cytotoxicity, sensitization, intradermal reaction, acute toxicity, hemolysis, pyrogenicity)
*Inquire to the contact details below if you wish to make a purchase.
Biomaster, Inc.

Cell strainer    Circulation circuit      Filtration circuit


Item Specifications
Name [Cell Washing Concentration System] Tubing Pump System
Model R-CS-S
Pump Flow rate range [Circulation pump] 20 - 500 [mL/min] (depending on fluid temperature)
[Feed pump / Drain pump] 20 - 350 [mL/min] (depending on fluid temperature)
Accuracy ±10 [%] Pump diameter φ80
No. of rollers 2 Clearance control Automatic adjustment
Applicable tube [Circulation pump] φ8.00 × φ12.00±0.15 [mm] (PVC based)
[Feed pump / Drain pump] φ6.40 × φ 9.50±0.15 [mm] (PVC based)
No. of units 2
Valve Type Ultrasonic Cutoff pressure 750 [mmHg] and more
Applicable tube φ3.50 × φ5.50 [mm] (PVC based)
No. of units 4
Pressure sensor Type Pressure transitor
Measuring range -750 - 750 [mmHg] (pressure gauge)
Accuracy ±5 [%]
Fitting Luer lock No. of units 2
Display Type TFT color LCD Effective display dimensions 116×87 [mm] (5.7 inch)
Operational method Touch panel (analog resistive film)
Outer dimensions W450×H695×D400 [mm]
Weight 43 [kg]
Power supply AC100 [V], 50/60 [Hz], 360 [W] (cable length: 2 [m])
Water proof protection class IPX1
Environment temperature Operation: 15～35[℃]／Transportation: 0～50[℃]
Environment humidity Operation: 35 - 85 [%] with no condensate / Transportation: 30 - 90 [%] with no condensate
Fluid temperature 15～35[℃]
External color SBY-S white (matte) / SBY blue (matte)

Contact

Bioprocess Equipment Division

TEL：81-48-471-9202 FAX：81-48-471-9203

SATAKE MultiMix Corporation

66, Niizo, Toda-shi, Saitama 335-0021, Japan
TEL: 81-48-433-8711 FAX: 81-48-433-8541

Copyright (C) 2017 SATAKE MultiMix Corporation
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