VMF-WSUB Reactor/TCS Controller | Bioreactors

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VMF-WSUB Reactor/TCS Controller

Equipped with weight management perfusion system
for commercial regenerative medicine production

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

When using iPS cells in 3D floating undifferentiated culture for regenerative medicine, efficient culture medium replacement is important. By stopping the mixing BioReactor for culture medium replacement, cells settle to the bottom of the culture tank having the ability of arbitrarily sticking together on the spot. This process results in uneven cell aggregation (sphere diameter), sometimes adversely affecting induced differentiation efficiency. When induced differentiation is the goal, single cells are discharged along with the culture medium, making it necessary to modify the inside of the culture tank so that they are not affixed to the surface. To satisfy these requirements, clogs must be avoided and culture medium discharge must be stabilized over a long period of time. A control system is an important part of maintaining continuous, precise culture medium replacement. The VMF-WSUB / TCS system has achieved these goals and features specifications that are capable of actual production.

A perfusion system that makes long-term,
continuous culturing possible!
Long-term, clog-free operation
from undifferentiated cultures to differentiation!

■Load cell for culture tank weight measurement

Mixing BioReactors experience frequent weight fluctuations, and this production system makes it possible to accurately gauge only the target weight inside the culture tank as well as culture medium replacement and perfusion control.

■Culture medium replacement holder and membrane set　MED-CH

This culture medium replacement holder and membrane set keeps the tank free from clogging for long periods of time and offers superb practical separation of cells and culture medium inside the tank.
There are several different types of membrane section depending on your purpose.

▼　VMF-W Reactor Specifications（Click to open details）

Item		Specifications
Name		VMF-W Reactor
Model		VMF-05W	VMF-3W
Temp. control device		Band heater (PID control) with over-temperature protection function (Max. 60 [℃])
Power transmission		Linear shaft drive non-sealing type
Gas supply type		Liquid surface gas absorption (option : sintered metal type)
Performance^*1	Temp. control range	Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. shaft stroke	40 [mm]
	Max. shaft speed	300 [mm/s]
Function	Temp. setting	Touch panel input, data output DC1 - 5 [V]
Function	Vertical motion setting	Touch panel input, data output DC0 - 5 [V]
Configuration	Power of band heater	60 [W] (Max. 60 [℃])	160 [W] (Max. 60 [℃])
	Power of motor	Max. power 800 [W]
	Mixing impellers	VM200	VM100+VM200
Culture tank	Dimensions	I.D. 94/87 x Depth 110 [mm]	I.D. 159/138 x Depth 202 [mm]
Culture tank	Culture operation volume	0.25 - 0.3 [L]	1.8 - 2.5 [L]
Usage condition		10 - 35 [℃]
Outer dimensions		W360×D485×H905 [mm]
Weight		Approx. 28 [kg]	Approx. 30 [kg]
Power supply		AC100 [V], 50/60 [Hz]
*1　The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

▼　S-BOX×TCS Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×TCS
Control	pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump ON/OFF
Display range/accuracy	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]		Display accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	In-vessel weight		0 - 6118 [g]
	In-vessel weight		0 - 6118 [g]
	Supply balance weight W1		-3200.00 - 3200.00 [g]
	Supply balance weight W1		-3200.00 - 3200.00 [g]
	Discharge balance weight W2		-3200.00 - 3200.00 [g]
	Discharge balance weight W2		-3200.00 - 3200.00 [g]
	Supply pump speed P1		0.0 - 200.0 [min^-1]
	Supply pump speed P1		0.0 - 200.0 [min^-1]
	Discharge pump speed P2		0.0 - 200.0 [min^-1]
	Discharge pump speed P2		0.0 - 200.0 [min^-1]
Setting range	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	In-vessel weight		0 - 6118 [g]
	In-vessel weight		0 - 6118 [g]
	Supply balance weight W1		-3200.00 - 3200.00 [g]
	Supply balance weight W1		-3200.00 - 3200.00 [g]
	Discharge balance weight W2		-3200.00 - 3200.00 [g]
	Discharge balance weight W2		-3200.00 - 3200.00 [g]
	Supply pump speed P1		0.0 - 200.0 [min^-1]
	Supply pump speed P1		0.0 - 200.0 [min^-1]
	Discharge pump speed P2		0.0 - 200.0 [min^-1]
	Discharge pump speed P2		0.0 - 200.0 [min^-1]
Control type	pH (Hydrogen ion concentration)		ON/OFF control
	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: TIME, %)
			O₂PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
	Fed-batch control (1)		Intermittent culture medium replacement control by in-vessel weight and roller pump

			Continuous culture medium replacement control by in-vessel weight and roller pump

	Fed-batch control (2)		Intermittent culture medium replacement control by culture medium supply/discharge balance weight and roller pump

			Continuous culture medium replacement control by culture medium supply/discharge balance weight and roller pump

Data output	pH (Hydrogen ion concentration)		With data logger DC0 - 5 [V] (option : Load factor can be outputted.)		Accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)
	DO (Dissolved oxygen)
	DO (Dissolved oxygen)
	FL (O₂flow rate)
	FL (O₂flow rate)
	Mixing frequency
	Mixing frequency
	Temperature sensor		DC1 - 5 [V]
	Temperature sensor		DC1 - 5 [V]
	In-vessel weight		Digital input type
	In-vessel weight
	Supply/discharge balance weight
	Supply/discharge balance weight
	Supply/discharge pump speed
	Supply/discharge pump speed
MTA of outer surface	SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×D400×H538 [mm]　・　Approx. 26 [kg]
Usage conditions	Temperature	5 - 45 [℃]		Humidity	20 - 85［%］ RH (No condensation)
Sensors	Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company


	(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)


Utilities	Power supply	AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	O₂	Flow rate 20/50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	*All of N₂, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.