Products & Services

Products & Services

Bioreactors

VMF-WSUB Reactor/TCS Controller

Equipped with weight management perfusion system
for commercial regenerative medicine production

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VMF-WSUB Reactor/TCS Controller

Patent No. 4316251

When using iPS cells in 3D floating undifferentiated culture for regenerative medicine, efficient culture medium replacement is important. By stopping the mixing BioReactor for culture medium replacement, cells settle to the bottom of the culture tank having the ability of arbitrarily sticking together on the spot. This process results in uneven cell aggregation (sphere diameter), sometimes adversely affecting induced differentiation efficiency. When induced differentiation is the goal, single cells are discharged along with the culture medium, making it necessary to modify the inside of the culture tank so that they are not affixed to the surface. To satisfy these requirements, clogs must be avoided and culture medium discharge must be stabilized over a long period of time. A control system is an important part of maintaining continuous, precise culture medium replacement. The VMF-WSUB / TCS system has achieved these goals and features specifications that are capable of actual production.

A perfusion system that makes long-term,
continuous culturing possible!
Long-term, clog-free operation
from undifferentiated cultures to differentiation!

Load cell for culture tank weight measurement

Load cell for culture tank weight measurement

Mixing BioReactors experience frequent weight fluctuations, and this production system makes it possible to accurately gauge only the target weight inside the culture tank as well as culture medium replacement and perfusion control.

Culture medium replacement holder and membrane set MED-CH

Culture medium replacement holder and membrane set MED-CH

This culture medium replacement holder and membrane set keeps the tank free from clogging for long periods of time and offers superb practical separation of cells and culture medium inside the tank.
There are several different types of membrane section depending on your purpose.


Item Specifications
Name VMF-W Reactor
Model VMF-500W VMF-3000W
Temperature control method Band heater (PID control) with anti-excessive temperature rise function (Max. 60/80 [℃])
Power transmission Linear shaft drive non-sealing type
Gas supply device Sintered metal type *Optional
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3 [℃] (37 [℃])
Max. up/down motion amplitude 40 [mm]
Max. impeller speed 300 [mm/s]
Function Temperature setting Digital setting, data output 1 - 5 [V]
Up/down motion setting Touch panel input, data output 0 - 10 [V]
Configuration Band heater 40 [W] (anti-excessive temperature rise: 60[℃]) 160 [W] (anti-excessive temperature rise: 80[℃])
Mixer motor Max. power 800 [W]
Mixing impellers [Standard] VM200 [Standard] VM100+VM200
Culture tank Dimensions I.D. 94/87 x Depth 110 [mm] I.D. 159/138 x Depth 202 [mm]
Total capacity 0.5[L] 3[L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm]
Weight approx. 28[kg] approx. 30[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×TCS
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
In-tank weight 0~6118 [g]
Supply balance weight W1 -3200.00~3200.00 [g]
Discharge balance weight W2 -3200.00~3200.00 [g]
Supply pump speed P1 0.0~200.0 [min-1]
Discharge pump speed P2 0.0~200.0 [min-1]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL (O2 flow rate) 0.00~20.00 [mg/L]
In-tank weight 0~6118 [g]
Supply balance weight W1 -3200.00~3200.00 [g]
Discharge balance weight W2 -3200.00~3200.00 [g]
Supply pump speed P1 0.0~200.0 [min-1]
Discharge pump speed P2 0.0~200.0 [min-1]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: time, %)
FL (O2 flow rate) PI control (slope set method: time, %)
Fed-batch control (1) Intermittent culture medium replacement control by in-tank weight and roller pump
Continuous culture medium replacement control by in-tank weight and roller pump
Fed-batch control (2) Intermittent culture medium replacement control by culture medium supply/discharge balance weight and roller pump
Continuous culture medium replacement control by culture medium supply/discharge balance weight and roller pump
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
*Load factor can be put out. (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL (O2 flow rate)
Mixing frequency
Temperature With data logger DC 1 - 5 [V]
In-tank weight With data logger
Digital input type
Supply/discharge balance weight
Supply/discharge pump speed
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×H538×D400 [mm] ・ approx. 26[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
N2 Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.