VMF-WSUB Reactor/TCS Controller | Bioreactors

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VMF-WSUB Reactor/TCS Controller

Equipped with weight management perfusion system
for commercial regenerative medicine production

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

Patent No. 4316251

When using iPS cells in 3D floating undifferentiated culture for regenerative medicine, efficient culture medium replacement is important. By stopping the mixing BioReactor for culture medium replacement, cells settle to the bottom of the culture tank having the ability of arbitrarily sticking together on the spot. This process results in uneven cell aggregation (sphere diameter), sometimes adversely affecting induced differentiation efficiency. When induced differentiation is the goal, single cells are discharged along with the culture medium, making it necessary to modify the inside of the culture tank so that they are not affixed to the surface. To satisfy these requirements, clogs must be avoided and culture medium discharge must be stabilized over a long period of time. A control system is an important part of maintaining continuous, precise culture medium replacement. The VMF-WSUB / TCS system has achieved these goals and features specifications that are capable of actual production.

A perfusion system that makes long-term,
continuous culturing possible!
Long-term, clog-free operation
from undifferentiated cultures to differentiation!

■Load cell for culture tank weight measurement

Mixing BioReactors experience frequent weight fluctuations, and this production system makes it possible to accurately gauge only the target weight inside the culture tank as well as culture medium replacement and perfusion control.

■Culture medium replacement holder and membrane set　MED-CH

This culture medium replacement holder and membrane set keeps the tank free from clogging for long periods of time and offers superb practical separation of cells and culture medium inside the tank.
There are several different types of membrane section depending on your purpose.

▼　VMF-W Reactor Specifications（Click to open details）

Item		Specifications
Name		VMF-W Reactor
Model		VMF-500W	VMF-3000W
Temperature control method		Band heater (PID control) with anti-excessive temperature rise function (Max. 60/80 [℃])
Power transmission		Linear shaft drive non-sealing type
Gas supply device		Sintered metal type *Optional
Performance	Temp. control range	Room temperature + 5 ～ 20℃ (normally set to 37℃)
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. up/down motion amplitude	40 [mm]
	Max. impeller speed	300 [mm/s]
Function	Temperature setting	Digital setting, data output 1 - 5 [V]
Function	Up/down motion setting	Touch panel input, data output 0 - 10 [V]
Configuration	Band heater	40 [W] (anti-excessive temperature rise: 60[℃])	160 [W] (anti-excessive temperature rise: 80[℃])
	Mixer motor	Max. power 800 [W]
	Mixing impellers	[Standard] VM200	[Standard] VM100+VM200
Culture tank	Dimensions	I.D. 94/87 x Depth 110 [mm]	I.D. 159/138 x Depth 202 [mm]
Culture tank	Total capacity	0.5[L]	3[L]
Environment temp. range		10～35[℃]
Outer dimensions		W360×H905×D485 [mm]
Weight		approx. 28[kg]	approx. 30[kg]
Power supply		AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

▼　S-BOX×TCS Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×TCS
Display range/accuracy	pH (Hydrogen ion concentration)		0.00～14.00 [pH]		Display accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)		0.00～20.00 [mg/L]
	FL (O₂flow rate)		0.00～20.00 [mL/min]
	In-tank weight		0～6118 [g]
	Supply balance weight W1		-3200.00～3200.00 [g]
	Discharge balance weight W2		-3200.00～3200.00 [g]
	Supply pump speed P1		0.0～200.0 [min^-1]
	Discharge pump speed P2		0.0～200.0 [min^-1]
Setting range	pH (Hydrogen ion concentration)		0.00～14.00 [pH]
	DO (Dissolved oxygen)		0.00～10.00 [mg/L]
	FL (O2 flow rate)		0.00～20.00 [mg/L]
	In-tank weight		0～6118 [g]
	Supply balance weight W1		-3200.00～3200.00 [g]
	Discharge balance weight W2		-3200.00～3200.00 [g]
	Supply pump speed P1		0.0～200.0 [min^-1]
	Discharge pump speed P2		0.0～200.0 [min^-1]
Control output	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: time, %)
	FL (O2 flow rate)		PI control (slope set method: time, %)
	Fed-batch control (1)		Intermittent culture medium replacement control by in-tank weight and roller pump
	Fed-batch control (1)		Continuous culture medium replacement control by in-tank weight and roller pump
	Fed-batch control (2)		Intermittent culture medium replacement control by culture medium supply/discharge balance weight and roller pump
	Fed-batch control (2)		Continuous culture medium replacement control by culture medium supply/discharge balance weight and roller pump
Data output	pH (Hydrogen ion concentration)		With data logger DC 0 - 5 [V] *Load factor can be put out. (optional)		Accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)
	FL (O2 flow rate)
	Mixing frequency
	Temperature		With data logger DC 1 - 5 [V]
	In-tank weight		With data logger Digital input type
	Supply/discharge balance weight
	Supply/discharge pump speed
Casing	SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×H538×D400 [mm]　・　approx. 26[kg]
Environment conditions	Temperature	5～45 [℃]	Humidity	20 - 85% RH (with no condensate)
Sensors	Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Sensors	Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities	Power supply	AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.