VerSus Reactor | Bioreactors

Products & Services

List of products

Catalogs

Mixers

Small machines

Water treatment

Petrochemistry

Food & beverage

Medicines

Bio & regenerative medical techniques

Paints

Paper manufacturing

For experiments

Super-mix Series

Classifier

Bioreactors

Realization of Industrialization / Commercial Production / Scalability

Microbial culture
HSF Reactor

Animal cell culture
MRF Reactor

Animal cell culture
MRF-RB Reactor

Animal cell culture
VMF Reactor

Animal cell culture
VerSus Reactor

Regenerative medicine
VMF-WSUB Reactor/TCS

Single-use BioReactor

iPS cell culture
HiD4×4

Fittings/Options

Solutions

Development of exclusive mixers・
and impellers by purpose of use

Contracted numerical calculation

Evaluation of contracted cell culture

Classification performance evaluation

Environmental testing equipment

BioReactors

HSF Reactor
MRF Reactor
MRF-RB Reactor
VMF Reactor
VerSus Reactor
VMF-WSUB
Reactor/TCS
SingleUse
BioReactor
HiD4x4
Fittings/Options

VerSus Reactor

Collaboration of VMF Reactor/SPG membrane sparger

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The VerSus Reactor is a new and innovative BioReactor for animal cell culture in which the technology of “SPG membrane sparger*” by which micro-bubbles with extremely high homogeneity can be generated is fused with a VMF Reactor. Through an efficient oxygen supply by the SPG membrane sparger, oxygen can be supplied efficiently without putting stress on the animal cells in the culture tank. Also, due to a decline in the DO air flow rate, a foam layer can be prevented. With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

SPG membrane sparger

The micro-bubbles can be homogeneously dispersed inside the tank.

*SPG membrane sparger has been developed jointly by MIYAZAKI PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

We promise further improvement in production efficiency with the “VerSus Reactor” which is a collaboration of the “VMF Reactor” that controls the physical action in the cell culture tank and “SPG membrane sparger”, which is an excellent micro bubble technology.

■Culture example

Comparison of cell viability
by CHO cells

The growth curve of CHO cells based on the presence of SPG membrane sparger is shown by using the VMF reactor. It is understood that the culture results improves significantly by using the SPG membrane sparger. Due to micro-bubbles having extremely high homogeneity, the high gas absorption performance significantly reduces the DO air flow rate, which is the cause of formation of a foam layer, without disturbing the weak flow. VerSus reactor has a significantly improved total performance.

Comparison of production of
antibodies by CHO cells

The result* of scale-up from the control quantity of 8 l to 150 l by using CHO cells having high dependability on the shear stress is shown below. Here, the actually commercially-available production of antibodies is undertaken. As a result, in the 150-l scale-up condition, either almost the same or more volume of production of antibodies than the control quantity of 8 l was obtained. This scale-up is performed by the numerical fluid calculation at a constant shear factor, and its advantage in the combined use of computational fluid dynamics and cell culture has been proved.
* The result of joint research with JGC Corporation

▼　VerSus Reactor Specifications（Click to open details）

Item		Specifications
Name		VerSus Reactor
Model		VSR-05	VSR-1	VSR-3	VSR-10
Temp. control device		Band heater (PID control) with over-temperature protection function (Max. 80 [℃])
Power transmission		Linear shaft drive non-sealing type
Gas supply type		Shirasu porous glass (SPG) membrane type　(additional option : sintered metal type)
Performance^*1	Temp. control range	Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. shaft stroke	40 [mm]
	Max. shaft speed	300 [mm/s]
Function	Temp. setting	Touch panel input, data output DC1 - 5 [V]
Function	Vertical motion setting	Touch panel input, data output DC0 - 5 [V]
Configuration	Power of band heater	60 [W]	105 [W]	160 [W]	480 [W]
	Power of motor	Max. power 800 [W]
	Mixing impellers	VM200	VM100+VM200^*2
Culture tank	Dimensions	I.D. 90 x Depth 200 [mm]	I.D. 110 x Depth 169 [mm]	I.D. 140 x Depth 203 [mm]	I.D. 200 x Depth 360 [mm]
Culture tank	Culture operation volume	0.3 [L]	1.2 [L]	2.4 [L]	7 [L]
Usage condition		10 - 35 [℃]
Outer dimensions		W360×D485×H905 [mm]
Weight		Approx. 28 [kg]	Approx. 28 [kg]	Approx. 30 [kg]	Approx. 34 [kg]
Power supply		AC100 [V], 50/60 [Hz]
*1　The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.
*2　Depending on the Culture operation volume, the only mixing impeller may be only the VM200.

▼　S-BOX×10αⅡ Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×10αII
Control	pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump
Display range/accuracy	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]		Display accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
Setting range	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 10.00 [mg/L]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
	FL (O₂flow rate)		0.00 - 20.00 [mL/min]
Control type	pH (Hydrogen ion concentration)		ON/OFF control
	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: TIME, %)
			O₂PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
	FL (O₂flow rate)		PI control (slope set method: TIME, %)
Data output	pH (Hydrogen ion concentration)		With data logger DC0 - 5 [V] (option : Load factor can be outputted.)		Accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)
	DO (Dissolved oxygen)
	DO (Dissolved oxygen)
	FL (O₂flow rate)
	FL (O₂flow rate)
	Mixing frequency
	Mixing frequency
	Temperature sensor		DC1 - 5 [V]
	Temperature sensor		DC1 - 5 [V]
MTA of outer surface	SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×D400×H530 [mm]　・　Approx. 15 [kg]
Usage conditions	Temperature	5 - 45 [℃]	Humidity	20 - 85 [%] RH (No condensation)
Sensors	Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company
Sensors	(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd. )
Utilities	Power supply	AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [Mpa] connection port φ6 one touch tube fitting
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	*All of N₂, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.