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VerSus Reactor

Collaboration of VMF Reactor/SPG membrane sparger

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

US Patent No. 10799841, Japan Patent No. 6534665, Other patents have either being applied for or already registered

The VerSus Reactor is a new and innovative BioReactor for animal cell culture in which the technology of “SPG membrane sparger*” by which micro-bubbles with extremely high homogeneity can be generated is fused with a VMF Reactor. Through an efficient oxygen supply by the SPG membrane sparger, oxygen can be supplied efficiently without putting stress on the animal cells in the culture tank. Also, due to a decline in the DO air flow rate, a foam layer can be prevented. With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

SPG membrane sparger

The micro-bubbles can be homogeneously dispersed inside the tank.

*SPG membrane sparger has been developed jointly by MIYAZAKI PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

We promise further improvement in production efficiency with the “VerSus Reactor” which is a collaboration of the “VMF Reactor” that controls the physical action in the cell culture tank and “SPG membrane sparger”, which is an excellent micro bubble technology.

■Culture example

Comparison of cell viability
by CHO cells

The growth curve of CHO cells based on the presence of SPG membrane sparger is shown by using the VMF reactor. It is understood that the culture results improves significantly by using the SPG membrane sparger. Due to micro-bubbles having extremely high homogeneity, the high gas absorption performance significantly reduces the DO air flow rate, which is the cause of formation of a foam layer, without disturbing the weak flow. VerSus reactor has a significantly improved total performance.

Comparison of production of
antibodies by CHO cells

The result* of scale-up from the control quantity of 8 l to 150 l by using CHO cells having high dependability on the shear stress is shown below. Here, the actually commercially-available production of antibodies is undertaken. As a result, in the 150-l scale-up condition, either almost the same or more volume of production of antibodies than the control quantity of 8 l was obtained. This scale-up is performed by the numerical fluid calculation at a constant shear factor, and its advantage in the combined use of computational fluid dynamics and cell culture has been proved.
* The result of joint research with JGC Corporation

▼　VerSus Reactor Specifications（Click to open details）

Item		Specifications
Name		VerSus Reactor
Model		VSR-500	VSR-1500	VSR-3000	VSR-10000
Temperature control method		Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission		Linear shaft drive non-sealing type
Gas supply device		(1) SPG sparger type Included, (2) sintered metal type Optional
Performance	Temp. control range	Room temperature + 5 ～ 20℃ (normally set to 37℃)
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. up/down motion amplitude	40 [mm]
	Max. impeller speed	300 [mm/s]
Function	Temperature setting	Digital setting, data output 1 - 5 [V]
Function	Up/down motion setting	Touch panel input, data output 1 - 10 [V]
Configuration	Band heater	60 [W]	105 [W]	160 [W]	480 [W]
	Mixer motor	Max. power 800 [W]
	Mixing impellers	[Standard] VM200	[Standard] VM100+VM200
Culture tank	Dimensions	I.D. 90 x Depth 200 [mm]	I.D. 110 x Depth 169 [mm]	I.D. 140 x Depth 203 [mm]	I.D. 200 x Depth 360 [mm]
Culture tank	Total capacity	0.5[L]	1.5[L]	3[L]	10[L]
Environment temp. range		10～35[℃]
Outer dimensions		W360×H905×D485 [mm]
Weight		approx. 28[kg]	approx. 28[kg]	approx. 30[kg]	approx. 34[kg]
Power supply		AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

▼　S-BOX×10αⅡ Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×10αII
Display range/accuracy	pH (Hydrogen ion concentration)		0.00～14.00 [pH]		Display accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)		0.00～20.00 [mg/L]
	FL (O₂flow rate)		0.00～20.00 [mL/min]
Setting range	pH (Hydrogen ion concentration)		0.00～14.00 [pH]
	DO (Dissolved oxygen)		0.00～10.00 [mg/L]
	FL (O₂flow rate)		0.00～20.00 [mL/min]
Control output	pH (Hydrogen ion concentration)		ON/OFF control
	DO (Dissolved oxygen)		O₂addition/subtraction step control
			O₂and N₂ON/OFF control
			O₂PI control (slope set method: time, %)
	FL (O₂flow rate)		PI control (slope set method: time, %)
Data output	pH (Hydrogen ion concentration)		With data logger DC 0 - 5 [V] *Load factor can be put out. (optional)		Accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)
	FL (O₂flow rate)
	Mixing frequency
	Temperature		With data logger DC 1 - 5 [V]
Casing	SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation	Indoor tabletop type
Outer dimensions/weight	W350×H530×D400 [mm]　・　approx. 15[kg]
Environment conditions	Temperature	5～45 [℃]	Humidity	20 - 85% RH (with no condensate)
Sensors	Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Sensors	Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities	Power supply	AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
	N₂	Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	O₂	Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
	*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.