Products & Services

Products & Services

BioReactors

VerSus Reactor

Collaboration of VMF Reactor/SPG membrane sparger

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VerSus Reactor

Patent No. 4316251, Other patents have either being applied for or already registered

The VerSus reactor is a new and innovative BioReactor for animal cell culture in which the technology of “SPG membrane sparger*” by which micro-bubbles with extremely high homogeneity can be generated is fused with a VMF reactor. Through an efficient oxygen supply by the SPG membrane sparger, oxygen can be supplied efficiently without putting stress on the animal cells in the culture tank. Also, due to a decline in the DO air flow rate, a foam layer can be prevented. With the help of the dedicated controller “S-BOX × 10α”, changes in various parameters including PI control can be easily performed.

SPG membrane sparger

SPG membrane sparger
The micro-bubbles can be homogeneously dispersed inside the tank.

*SPG membrane sparger has been developed jointly by MIYAZAKI PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

We promise further improvement in production efficiency with the “VerSus Reactor” which is a collaboration of the “VMF Reactor” that controls the physical action in the cell culture tank and “SPG membrane sparger”, which is an excellent micro bubble technology.

Culture example

Culture example

Comparison of production of
antibodies by CHO cells

The growth curve of CHO cells based on the presence of SPG membrane sparger is shown by using the VMF reactor. It is understood that the culture results improves significantly by using the SPG membrane sparger. Due to micro-bubbles having extremely high homogeneity, the high gas absorption performance significantly reduces the DO air flow rate, which is the cause of formation of a foam layer, without disturbing the weak flow. VerSus reactor has a significantly improved total performance.

Comparison of production of
antibodies by CHO cells

The result* of scale-up from the control quantity of 8 l to 150 l by using CHO cells having high dependability on the shear stress is shown below. Here, the actually commercially-available production of antibodies is undertaken. As a result, in the 150-l scale-up condition, either almost the same or more volume of production of antibodies than the control quantity of 8 l was obtained. This scale-up is performed by the numerical fluid calculation at a constant shear factor, and its advantage in the combined use of computational fluid dynamics and cell culture has been proved.
* The result of joint research with JGC Corporation

■VerSus Reactor Specifications
Item Specifications
Product name VerSus Reactor
Model VSR-500 VSR-1500 VSR-3000 VSR-10000
Temperature control type Band heater method (PID control) with excessive temperature rise prevention function(MAX 80℃)
Linear-motion transmission method Linear shaft drive non-sealing type
Ventilation type Shirasu porous glass (SPG) membrane type
Performance Temperature regulation range Room temperature+5~20℃(normally set to 37℃)
Temperature regulation accuracy ±0.3℃(37℃)
Max. up/down motion amplitude 40mm
Max. impeller speed 300mm/s
Function Temperature setting Digital setting
Up/down motion setting Touch panel input
Configuration Band heater 150W
Mixing motor Max. output 800W
Mixing impellers Standard VM200 Standard VM100 + VM200
Standard Culture tank dimensions Inner diameter90
×200Depth [mm]
Inner diameter110
×169Depth [mm]
Inner diameter140
×203Depth [mm]
Inner diameter200
×360sDepth [mm]
Culture tank total capacity 0.5L 1.5L 3L 10L
Operating ambient temperature range 10~35℃
Outer dimensions 300W×485D×890H[mm] (When a fall prevention fitting has been attached、360W)
Weight Approx. 28kg Approx. 28kg Approx. 30kg Approx. 34kg
Rated power supply AC-100V, 50/60Hz

* The performance is the value at a room temperature of 20℃, rated power supply voltage 100 VAC 50 Hz, during no load.

■S-BOX×10αSpecifications
Item Specifications
Name Culture controller
Model S-BOX×10α
Display range / accuracy pH 0.00~14.00 [pH] Display accuracy:F.S±0.5%
DO(Dissolved oxygen) 0.00~10.00 [mg/L]
FL(O₂ flow rate) 0.00~20.00 [mL/min]
Control setting range pH 0.00~14.00 [pH]
DO(Dissolved oxygen) 0.00~10.00 [mg/L]
FL(O₂ flow rate) 0.00~20.00 [mL/min]
Control output pH ON/OFF control
DO(Dissolved oxygen) Flow rate addition/subtraction step control
FL(O₂ flow rate) PI control (slope set method: Time, %)
Record output pH Data logger included
DC 0~5 [V]
Output accuracy:F.S±0.5%
DO(Dissolved oxygen)
FL(O₂ flow rate)
Power supply AC 100 V, 50/60Hz
Case material SUS304 (No coating)
Indoor type, non-drip proof, non-explosion proof specification
Installation method Indoor tabletop type
Outer dimensions W260×H350×D300 [mm]
Ambient environment conditions Temperature 5~45 ℃
Humidity 20~85 %(No dew formation)
Utility Power supply 100 VAC electrical outlet × 2 (for controller unit and recorder)
O₂ Flow rate 20 ml/min or less Supply pressure 0.3 MPa
Connection port Φ6 tube one-touch joint
CO₂ Flow rate 50 ml/min or less Supply pressure 0.2 MPa
Connection port Φ6 tube one-touch joint
AIR Flow rate 150 ml/min or less Supply pressure 0.2 MPa
Connection port Φ6 tube one-touch joint

* O₂, CO₂, and AIR must all be dry gases that do not contain corrosive components, dust, and oil mist