Products & Services

Products & Services

BioReactors

VerSus Reactor

Collaboration of VMF Reactor/SPG membrane sparger

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VerSus Reactor

US Patent No. 10799841, Japan Patent No. 6534665, Other patents have either being applied for or already registered

The VerSus Reactor is a new and innovative BioReactor for animal cell culture in which the technology of “SPG membrane sparger*” by which micro-bubbles with extremely high homogeneity can be generated is fused with a VMF Reactor. Through an efficient oxygen supply by the SPG membrane sparger, oxygen can be supplied efficiently without putting stress on the animal cells in the culture tank. Also, due to a decline in the DO air flow rate, a foam layer can be prevented. With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

SPG membrane sparger

SPG membrane sparger
The micro-bubbles can be homogeneously dispersed inside the tank.

*SPG membrane sparger has been developed jointly by MIYAZAKI PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

We promise further improvement in production efficiency with the “VerSus Reactor” which is a collaboration of the “VMF Reactor” that controls the physical action in the cell culture tank and “SPG membrane sparger”, which is an excellent micro bubble technology.

Culture example

Culture example

Comparison of cell viability
by CHO cells

The growth curve of CHO cells based on the presence of SPG membrane sparger is shown by using the VMF reactor. It is understood that the culture results improves significantly by using the SPG membrane sparger. Due to micro-bubbles having extremely high homogeneity, the high gas absorption performance significantly reduces the DO air flow rate, which is the cause of formation of a foam layer, without disturbing the weak flow. VerSus reactor has a significantly improved total performance.

Comparison of production of
antibodies by CHO cells

The result* of scale-up from the control quantity of 8 l to 150 l by using CHO cells having high dependability on the shear stress is shown below. Here, the actually commercially-available production of antibodies is undertaken. As a result, in the 150-l scale-up condition, either almost the same or more volume of production of antibodies than the control quantity of 8 l was obtained. This scale-up is performed by the numerical fluid calculation at a constant shear factor, and its advantage in the combined use of computational fluid dynamics and cell culture has been proved.
* The result of joint research with JGC Corporation


Item Specifications
Name VerSus Reactor
Model VSR-500 VSR-1500 VSR-3000 VSR-10000
Temperature control method Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission Linear shaft drive non-sealing type
Gas supply device (1) SPG sparger type *Included, (2) sintered metal type *Optional
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3 [℃] (37 [℃])
Max. up/down motion amplitude 40 [mm]
Max. impeller speed 300 [mm/s]
Function Temperature setting Digital setting, data output 1 - 5 [V]
Up/down motion setting Touch panel input, data output 1 - 10 [V]
Configuration Band heater 60 [W] 105 [W] 160 [W] 480 [W]
Mixer motor Max. power 800 [W]
Mixing impellers [Standard] VM200 [Standard] VM100+VM200
Culture tank Dimensions I.D. 90 x Depth 200 [mm] I.D. 110 x Depth 169 [mm] I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]
Total capacity 0.5[L] 1.5[L] 3[L] 10[L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm]
Weight approx. 28[kg] approx. 28[kg] approx. 30[kg] approx. 34[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×10αII
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: time, %)
FL (O2flow rate) PI control (slope set method: time, %)
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
*Load factor can be put out. (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL (O2flow rate)
Mixing frequency
Temperature With data logger
DC 1 - 5 [V]
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×H530×D400 [mm] ・ approx. 15[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
N2 Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.