Products & Services

Products & Services

Bioreactors

A single-use BioReactor

VMF Reactor 50L/200L-SUB

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

A single-use BioReactor

Patent No. 4316251,
Other patent pending or registered

The lineup consists of models from 50 to 200 L (development planned for up to 1,000 L) with tabletop models from 500 mL to 10 L, which are self-standing from 50 L and up. This is the largest model in the standard VMF reactor series and a commercial production device that is perfectly scalable, offering an unprecedented starting size of 500 mL. The S-BOX × 200 controller is included as a standard, offering full control over DO, pH, temperature, in-bag pressure, etc., and is equipped with four embedded pumps in a series, a dedicated internal digital data logger, Satake data logger software, and a BioReactor data collection system, etc. It also supports computerized system validation, and documents are provided for qualification confirmation during the design stage, during installation, and during operation. We also provide support for clients who are not accustomed to creating user requested specifications. We guarantee just-in-time supply in small lots of locally-manufactured single-use bags for sterilization validation. We can meet various demands required for commercial production, so please feel free to contact us.

Bag for single use

Bag for single use

Patent No.6313635

●A completely hermetically sealed construction
 ensures a contamination-free environment.
●Since this is a 100% local product, we can
 always provide a stable supply even in small lots.
●Since sterilization has already been performed,
 you need not perform washing and sterilization
 at the time of use.
●The bag, impeller, shaft, and tube, etc. use
 materials conforming to USP Class VI
 (United States Pharmacopoeia Class 6).
●The DO/pH sensor is attached after high-pressure
 steam sterilizer in an autoclave.
●An optional single-use pressure sensor is also
 available.

Each sensor

Each sensor

Each sensor is attached to the culture tank as shown in the photo. The DO and pH sensors are attached using a Thermo Fisher Scientific bioreactor probe assembly with a sterile AseptiQuik Connector from CPC. The temperature sensor is installed inside a silicone tube sheath. It enables accurate temperature readings of production equipment without injecting water into the sheath.

CFD simulation analysis results

CFD simulation analysis results

Model

Flow velocity
distribution

Turbulent energy

Shear stress

Consideration of commercial production scale-up
and utilization of numerical fluid calculation


Item Specifications
Product name VMF Reactor
Model 50L-SUB*
Temperature control method Rubber heater (PID control) with anti-excessive temperature rise function
Power transmission Linear shaft drive non-sealing type
Gas supply device Shirasu porous glass (SPG) membrane type / sintered metal type
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3 [℃] (37 [℃])
Max. up/down motion amplitude 100 [mm]
Max. impeller speed 800 [mm/s]
Function Temperature setting Digital setting, data output 1 - 5 [V]
Up/down motion setting Touch panel input, data output 1 - 10 [V]
Configuration Rubber heater 1.5 [kW]
Mixer motor Max. output 800 [W]
Mixing impellers [Standard] VM100+VM200
Culture tank Dimensions I.D. 369 x Depth 650 [mm]
Total capacity 75[L]
Environment temp. range 10~35[℃]
Outer dimensions W780×H200×D800 [mm]
Weight Main unit approx. 135 [kg], Vessel ASSY approx. 42 [kg]
Power supply Power is supplied from controller S-BOX × 200
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.
*For 200L-SUB, please contact us for further information.

Item Specifications
Name Culture controller
Model S-BOX × 200
Display range/accuracy TH (Temperature) 0.0~150.0[℃] DO (Dissolved oxygen) 0.0~100.0[%]
Linear shaft (mixing) Stroke 0-100 [mm], Speed setting 0-800 [mm/s] FL (O₂ flow rate) 0.00~5.00[L/min]
pH (Hydrogen ion concentration) 0.00 - 14.00 [pH] Electronic balance (for culture medium supply) 0.1[g]~21[kg]
Setting range TH (Temperature) 0.0 - 60.0 [℃] DO (Dissolved oxygen) 0.0~100.0[%]
Linear shaft (mixing) Stroke 0-100 [mm], Speed setting 0-800 [mm/s] FL (O2 flow rate) 0.50~5.00[L/min]
pH (Hydrogen ion concentration) 0.00~14.00[pH] Electronic balance (for culture medium supply) 0.1[g]~21[kg]
Control output TH (Temperature) PI control (slope set method: time, %)
Linear shaft (mixing) In-place control by shaft driver
pH (Hydrogen ion concentration) ON/OFF control by addition of CO₂ gas and ON/OFF control with alkaline pump (Selectable)
DO (Dissolved oxygen) PI control (slope set method: time, %) Using mass flow controller
FL (O2 flow rate) PI control (slope set method: time, %)
FEED ON/OFF control with FEED pump
Culture medium supply / discharge Supply and discharge by electronic balance, culture medium supply, and discharge pump
Data output pH (hydrogen ion concentration), DO (dissolved oxygen), FL (O2flow rate), temperature, Mixing frequency *Load factor (optional) by data logger 10ch
Casing SUS304 #300 Buffing, indoor type, non-drip proof, non-explosion proof specification
Installation Indoor stand-alone type
Outer dimensions/weight Outer dimensions W550×H1000×D550 [mm] *Does not include protrusions Approx. 90 [kg]
Environment conditions Temperature 5 - 45 [℃] Humidity 20 - 85%RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply 1 [Φ] 200 [V] 50/60 [Hz] 30 [A] (2P + E, electrical outlet for 30 A hook-type plug) × 1
O2 Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
CO2 Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
AIR Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

SingleUse System Up

Single-use Bottles/Single-use Bags

In biomedical products, regenerative medicine, and other such areas, single-use products are preferable from the perspectives of increased productivity and prevention of contamination. At SATAKE, we provide single-use products with total capacities ranging from 500mL to 200L (with plans to develop products of up to 1000L in capacity) to meet a wide range of needs. Our products achieve cell culture in a scalable manner from the laboratory to actual production.

Single-use Bottles

* The 500mL single-use bottle is compatible with the "VMF Reactor VMF-500 and HiD".
* The 3L single-use bottle is compatible with the" VMF Reactor VMF-3000" and also with 2 to 8 optional devices connected.

single-use bag

* The 500mL single-use bag is compatible with the "VMF Reactor VMF-10000 and VMF Reactor VMF-50L / 200L-SUB".

single-use bag

Various materials used in impellers,
bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times.

Proposal of application development
and customization

The single-use bag product lineup has a maximum capacity of 200 L, but we can also support bags of 500 to 1000 L capacity according to the application.
If you have a request for customized products that match the equipment, please contact us.

Sterilized standard bottles and bags

We prepare hard bottles and bags in the range of 500 mL to 200L. Since EOG sterilization for hard bottles, γ sterilization for back has already been performed, you can start using these products as soon as you receive them. We have prepared certification for each lot.
Various testing has been conducted and conformity confirmed on each material used so you can use the products immediately without worry.


Item Specifications
Name Single-use Bottles Single-use Bags (3D) Single-use Bags (2D)
Model VMH-500 VMH-1000/3000 VMB-10 VMB-50 VMB-200 SCB-10 SCB-50
Total capacity 500[mL] 3[L] 10[L] 50[L] 200[L] 10[L] 50[L]
Dimensions I.D. 94 (87) [mm] I.D. 159 (138) [mm] I.D. 206 [mm] I.D. 369 [mm] I.D. 590 [mm] 560×330 [mm]
*Outer dimensions
740×705 [mm]
*Outer dimensions
Depth 110 [mm] Depth 202 [mm] Depth 360 [mm] Depth 650 [mm] Depth 1015 [mm]
Ports Gas inlet, Gas outlet, sampling, temperature sensor, DO sensor, pH sensor, culture medium replacement, inoculation Culture medium inlet/outlet, sampling, substrate inlet, cell inlet, air inlet/outlet, O2inlet, temperature sensor, DO sensor, pH sensor Gas inlet, Gas outlet, harvest, sampling, cell inlet, culture medium inlet, spare*
*Spare port only on SCB-50

Multipurpose Bag

This bag can be used for various purposes such as supply, discharge, and reagent use during continuous culture medium replacement. It can also be customized, so please feel free to inquire. γ-ray sterilization is employed to make this bag suitable for use with pharmaceuticals.

Multipurpose Bag

Bottles/Bags with Single-use Sensors

Single-use sensors (DO, pH) made by Mettler Toledo - well-known for culture-related products - are pre-attached to the bottle/bag. These can be used without autoclave sterilization, enabling preparation time to be shortened. They are also perfect for contamination prevention.

Bottles/Bags with Single-use Sensors