Products & Services

Products & Services

Bioreactors

MRF Reactor

Rotating type mixer for cell culture equipped with an MR210Bio impeller as a standard

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

MRF Reactor
The MRF Reactor is a BioReactor for laboratory use that aim at actual production and scaling up. In addition to a “Simple shape with excellent cleaning ability” that is important for the actual equipment, the “MR210Bio impeller used exclusively for cell culture” that exhibits excellent mixing and homogenizing performance is provided as a standard, and therefore, similar culture results as during laboratory experiments can be reproduced during scale-up too. Moreover, fluid surface fluctuation (fed-batch culture) is supported, because of which the same mixing performance is exhibited no matter how much the liquid volume. A wide range of liquid volumes from small volumes like 1.5 L to large volumes of 20,000 l can be supported.
With the help of the dedicated controller “S-BOX × 10α II”, changes in various parameters including PI control can be easily performed.

Mixing Impeller Super-mix MR210Bio Used Exclusively for Cell Culture

Mixing Impeller Super-mix MR210Bio Used Exclusively for Cell Culture

・Exhibits high mixing performance at low shear
・Simple shape with excellent washing ability
・Completely compatible with fluid surface fluctuation
・The same culture results as in the laboratory can be reproduced during scale-up too.

An environment that is best suited for cell culture is created through excellent mixing performance.

Flow condition inside culture tank

Flow condition inside culture tank

CFD Simulation Analysis Result

The “MR210Bio” impeller developed exclusively for cell culture produces a high circulating flow that runs from the low-pressure area at the bottom of the blades, where the mixing homogeneity inside the tank is high, toward the top of the tank. As a result of this feature, a high homogeneity and fluidity are obtained even at low power and low rotation, and at the same time, the basic flow pattern does not change even when the fluid surface fluctuates. It is known as an impeller used exclusively for cell culture that adapts to unsteadiness, which was not seen in the past in any impeller, and is suitable for operation under conditions where the fluid surface fluctuates as a result of fed-batch culture. A high circulation performance can be seen from the CFD simulation analysis results.

Culture example

Culture example

Comparison of the cell viability in the later stage of the culture by CHO cells

Here we show the cell viability in the later stage of the culture under the batch cell culture condition for the turbine type (flat turbine) and wide paddle type (elephant ear) impellers used in the conventional rotating type BioReactor. It can be confirmed that the MRF reactor maintains a higher cell viability than the other conventional impellers in the later stage of the culture. A characteristic of the MR210Bio that has a low shearing action and high mixing homogeneity performance is the realization of scale-up to a large-size reactor through the combined use of numerical fluid calculation.


Item Specifications
Name MRF Reactor
Model MRF-3000 MRF-8000 (10% dish bottom head) MRF-10000
Temperature control method Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission Magnet drive non-sealing type
Gas supply device Shirasu porous glass (SPG) membrane type / sintered metal type
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3[℃](37[℃])
Rotation speed range 5~200[min-1]
Function Temp. setting Digital setting, data output 1 - 5 [V]
Speed setting Touch panel input, data output 0 - 10 [V]
Configuration Band heater 160[W] 480[W] 480[W]
Mixer motor Max. output 100 [W]
Mixing impellers [Standard] MR210Bio impeller
Culture tank Dimensions I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 330 [mm] I.D. 200 x Depth 360 [mm]
Total capacity 3 [L] 8 [L] (10% dish bottom head) 10 [L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm]
Weight approx. 30[kg] approx. 34[kg] approx. 34[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×10αII
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: time, %)
FL (O2flow rate) PI control (slope set method: time, %)
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
*Load factor can be put out. (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL (O2flow rate)
Mixing frequency
Temperature With data logger
DC 1 - 5 [V]
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×H530×D400 [mm] ・ approx. 15[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
N2 Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

Item Specifications
Name Culture controller
Model S-BOX×10 Simple
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) ON/OFF control
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W260×H350×D300 [mm] ・ approx. 12[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessory: Automatic System Research Co., Ltd. Optical DO sensor
+ Mettler Toledo company pH sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.