Products & Services

Products & Services

Bioreactors

MRF Reactor

Rotating type mixer for cell culture equipped with an MR210Bio impeller as a standard

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

MRF Reactor
The MRF reactor is a BioReactor for laboratory use that aim at actual production and scaling up. In addition to a “Simple shape with excellent cleaning ability” that is important for the actual equipment, the “MR210Bio impeller used exclusively for cell culture” that exhibits excellent mixing and homogenizing performance is provided as a standard, and therefore, similar culture results as during laboratory experiments can be reproduced during scale-up too. Moreover, fluid surface fluctuation (fed-batch culture) is supported, because of which the same mixing performance is exhibited no matter how much the liquid volume. A wide range of liquid volumes from small volumes like 1.5 L to large volumes of 20,000 l can be supported.
With the help of the dedicated controller “S-BOX × 10α”, changes in various parameters including PI control can be easily performed.

Mixing Impeller Super-mix MR210Bio Used Exclusively for Cell Culture

Mixing Impeller Super-mix MR210Bio Used Exclusively for Cell Culture

・Exhibits high mixing performance at low shear
・Simple shape with excellent washing ability
・Completely compatible with fluid surface fluctuation
・The same culture results as in the laboratory can be reproduced during scale-up too.

An environment that is best suited for cell culture is created through excellent mixing performance.

Flow condition inside culture tank

Flow condition inside culture tank

CFD Simulation Analysis Result

The “MR210Bio” impeller developed exclusively for cell culture produces a high circulating flow that runs from the low-pressure area at the bottom of the blades, where the mixing homogeneity inside the tank is high, toward the top of the tank. As a result of this feature, a high homogeneity and fluidity are obtained even at low power and low rotation, and at the same time, the basic flow pattern does not change even when the fluid surface fluctuates. It is known as an impeller used exclusively for cell culture that adapts to unsteadiness, which was not seen in the past in any impeller, and is suitable for operation under conditions where the fluid surface fluctuates as a result of fed-batch culture. A high circulation performance can be seen from the CFD simulation analysis results.

Culture example

Culture example

Comparison of the cell viability in the later stage of the culture by CHO cells

Here we show the cell viability in the later stage of the culture under the batch cell culture condition for the turbine type (flat turbine) and wide paddle type (elephant ear) impellers used in the conventional rotating type BioReactor. It can be confirmed that the MRF reactor maintains a higher cell viability than the other conventional impellers in the later stage of the culture. A characteristic of the MR210Bio that has a low shearing action and high mixing homogeneity performance is the realization of scale-up to a large-size reactor through the combined use of numerical fluid calculation.

■MRF Reactor Specifications
Item Specifications
Product name MRF Reactor
Model MRF - 3000 MRF - 10000
Temperature control type Band heater method (PID control) with excessive temperature rise prevention function
Rotating type Magnet drive non-sealing type
Ventilation type Shirasu porous glass membrane type / Sintered metal type
Performance Temperature regulation range Room temperature+5~20℃(normally set to 37℃)
Temperature regulation accuracy ±0.3℃(37℃)
Rotation range 5~200rpm
Function Temperature setting Digital setting
RPM setting Touch panel input
Configuration Band heater 150W
Mixing motor Max. output 100W
Mixing impellers Standard MR210Bio impeller
Standard Culture tank dimensions Inner diameter140×203Depth [mm] Inner diameter200×360Depth [mm]
Culture tank total capacity 3L 10L
Operating ambient temperature range 10~35℃
Outer dimensions 300W×485D×890H [mm] When a fall prevention fitting has been attached
Weight Approx. 30kg Approx. 34kg
Rated power supply AC-100V, 50/60Hz

* The performance is the value at a room temperature of 20℃, rated power supply voltage 100 VAC 50 Hz, during no load.

■S-BOX×10αSpecifications
Item Specifications
Name Culture controller
Model S-BOX×10α
Display range / accuracy pH 0.00~14.00 [pH] Display accuracy:F.S±0.5%
DO(Dissolved oxygen) 0.00~10.00 [mg/L]
FL(O₂ flow rate) 0.00~20.00 [mL/min]
Control setting range pH 0.00~14.00 [pH]
DO(Dissolved oxygen) 0.00~10.00 [mg/L]
FL(O₂ flow rate) 0.00~20.00 [mL/min]
Control output pH ON/OFF control
DO(Dissolved oxygen) Flow rate addition/subtraction step control
FL(O₂ flow rate) PI control (slope set method: Time, %)
Record output pH Data logger included
DC 0~5 [V]
Output accuracy:F.S±0.5%
DO(Dissolved oxygen)
FL(O₂ flow rate)
Power supply AC 100 V, 50/60Hz
Case material SUS304 (No coating)
Indoor type, non-drip proof, non-explosion proof specification
Installation method Indoor tabletop type
Outer dimensions W260×H350×D300 [mm]
Ambient environment conditions Temperature 5~45 ℃
Humidity 20~85 %(No dew formation)
Utility Power supply 100 VAC electrical outlet × 2 (for controller unit and recorder)
O₂ Flow rate 20 ml/min or less Supply pressure 0.3 MPa
Connection port Φ6 tube one-touch joint
CO₂ Flow rate 50 ml/min or less Supply pressure 0.2 MPa
Connection port Φ6 tube one-touch joint
AIR Flow rate 150 ml/min or less Supply pressure 0.2 MPa
Connection port Φ6 tube one-touch joint

* O₂, CO₂, and AIR must all be dry gases that do not contain corrosive components, dust, and oil mist