Products & Services

Products & Services

Bioreactors

HSF Reactor

High-performance BioReactor with the high-efficiency turbine HS100 / high-discharge axial flow impeller HR100 as a standard

High-Efficiency Turbine, In-Tank Coil Heat Transmission Specs, Dedicated for Actual Machine Scale-up

HSF Reactor
The HSF Reactor is a jar fermenter designed for comparatively low-viscosity microbe cultures, plant-based cultures, fungal-based cultures with high viscosity that show non-Newtonian properties, E. coli cultures, etc., in which target cells and bacterial cells have a comparatively strong shear factor, and cultures that require high OTR. It comes with the high-efficiency turbine HS100 and high-discharge axial flow impeller HR100 as a standard. Optimization is possible for scaling up to large-capacity tasks when paired with the S-BOX series controller for culture control.
An optional cooling chiller can be attached, and when using a heat transfer coil type similar to the actual machine, you can achieve conditions that are closer to the actual machine. As a specialty manufacturer of mixing device with the only dedicated mixing technology laboratory in Japan, we can apply final optimizations for actual machine and customize the device according to the physical properties of its contents.

High performance turbine impeller and axial flow impeller are standard specifications

HS100 turbine

HS100 turbine

The HS100 turbine achieves an extremely low power number (Power number ratio to 6FT: approx. 65% reduction). Liquid fluidizing action is improved compared to conventional turbines, along with high-performance gas absorption (KLa). Impeller lift at low power creates more focused flow, utilizing pressure gradients and fluctuations of the discharge field to produce powerful shearing and destructive action. When paired with the high-discharge type HR100 Impeller, this impeller achieves overall incredibly highly efficient in-tank liquid fluidizing action, shearing, and destructive action (gas dispersion).

Comparison of gas absorption performance (kLa) CFD flow analysis result near the blade (blade cross section)

Comparison of gas
absorption performance (kLa)

6 flat
turbine

HS100
turbine

CFD flow analysis result near the blade
(blade cross section)




HR100 Impeller

HR100 Impeller

In addition to considerations regarding the plane shape of the impeller, the angle of attack, and the camber ratio, the HR100 Impeller features a multistage curved structure, and suppressing separation on the rear surface of the impeller results in an energy-saving, low-shear type axial flow impeller that boasts high discharge performance at low power. This product excels at liquid-liquid mixing, solid-liquid dispersion, uniform suspension of particles that are fragile and lightweight, emulsified micro-capsules (latex, etc.), and is effective in combinations that utilize superior axial fluidizing action in multi-stage mixing.

Flow pattern of the HR100 Impeller Discharge performance by LDV (compared with conventional 4PP model)

Flow pattern of the HR100 Impeller

Discharge performance by LDV
(compared with conventional 4PP model)

Example of 200 kL Actual Machine CFD
Simulation Analysis

Example of 200 kL Actual Machine CFD Simulation Analysis

Conventional model: 6-bladed flat turbine triple stage

Satake model: HR100 double stage + HS100 single stage

Gas dispersion comparison results via multiphase flow model (Euler-Euler)
of 200 kL culture tank in CFD simulation

When using the conventional 6-bladed flat turbine in multi-stage within a culture tank, the flow is divided creating conditions that are not favorable to uniform dispersion inside the culture tank. With this in mind, a new, high-performance BioReactor is created by utilizing the flow action in the axial direction of high-discharge axial flow impeller HR100 on the top of the tank in combination with high-dispersion turbine HS100 on the bottom of the tank for gas dispersion action, resulting in uniform dispersion inside the culture tank and high-performance gas absorption. HSF reactors are suitable for lab-scale consideration from 3 to 10 L, and can also be scaled up to production machines (large capacity) at the same requirements.

Super-mix® HS124ND, HS134ND Turbine

Super-mix®HS124ND, HS134ND Turbine

HS124ND
Patent No. 6159577

HS134ND
Patent No. 6159577

Ultra high-efficiency turbines HS124ND/HS134ND boast gas absorption performance that exceeds even that of the high-efficiency turbine HS100. The upper and lower impellers create effective discharge action, achieving a high level of gas absorption performance and required OTR. Technology that delivers the world's highest gas absorption performance and required OTR is available at laboratory scale in 200 - 300 kL-class actual machines.

SATAKE Sparger

SATAKE Sparger

Satake Spargrt Patent No. 6189202

The Satake Sparger is designed with gas discharge direction in mind so that aerated gas passes near the impeller, which is where the shearing field is located. Also, a stationary impeller is attached to the ring, and increasing the velocity gradient of the flow improves shearing and destructive action. Furthermore, the stationary impeller strengthens the discharge action. These actions efficiently disperse gases.

200kL-F culture tank simulation results

Conventional Sparger

SATAKE Sparger

200kL-F culture tank simulation results
Gas-liquid multiphase flow gas fraction analysis

The Satake Sparger is effective when paired with the HS100. The synergistic effect with the optimized mixing impeller significantly improves the gas dispersion action, increasing gas absorption performance. (According to our test results)


Item Specifications
Name HSF Reactor
Model HSF-3000 HSF-10000
Temperature control method Band heater (PID control) with anti-excessive temperature rise function
(Max. 80℃)+ [Option] In-tank heat transfer coil
Power transmission Magnet drive non-sealing type
Gas supply device [Standard] Ring sparger / [Option] SATAKE sparger
Perform
ance
Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3[℃](37[℃])
Rotation speed range 5~1500[min-1]
Function Temp. setting Digital setting, data output 1 - 5 [V]
Speed setting Touch panel input, data output 0 - 10 [V]
Configur
ation
Band heater 160[W] 480[W]
Mixer motor Max. output 100 [W] Max. output 400 [W]
Mixing impellers [Standard] HS100 + HR100 impellers
Culture tank Dimensions I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]
Total capacity 3[L] 10[L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm] W360×H980×D485 [mm]
Weight approx. 30[kg] approx. 34[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX × 10 MC
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL(O2 0.4~10.0 [L/min]
FL(AIR) 0.4~20.0 [L/min]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL(O2 0.4~10.0 [L/min]
FL(AIR) 0.4~20.0 [L/min]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) ON/OFF control
AF (antifoam) ON/OFF control
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL(O2
FL(AIR)
AF
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W260×H400×D320 [mm] ・ approx. 15[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for control unit and recorder)
O2 Flow rate 10 [L/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR (for pH) Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR (for DO) Flow rate 20 [L/min] or lower (controlled with mass flow controller) Supply pressure 0.2 [MPa] Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

Item Specifications
Name Culture controller
Model S-BOX×10 Simple
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen)
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W260×H350×D300 [mm] ・ approx. 12[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessory: Automatic System Research Co., Ltd. Optical DO sensor
+ Mettler Toledo company pH sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.