Products & Services

Products & Services

Bioreactors

HiD 4×4

3D floating iPS cell differentiation induction BioReactorr

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

HiD 4×4

Patent No. 4316251, Other patents have either being applied for or already registered

The HiD 4 × 4 Reactor is the world’s first “Single-use mixer for 3D floating cell cultures” used exclusively for iPS cell differentiation induction. A BioReactor that achieves a high-volume production of uniform and homogeneous iPS cells is indispensable not only in regenerative medicine, but also in the “Use of human iPS cells in drug development” that aims at industrialization and commercial production. By promoting joint research and development with excellent research institutes or companies in Japan, we have succeeded in commercialization an iPS cell differentiation induction BioReactor for the first time in the world. 4, 8, 12, and 16 (or even more) such BioReactors can be controlled in a consolidated manner, and these are also best suited for screening. Moreover, we have also taken into consideration the adaptation to ES cells while performing development. With the help of the dedicated controller “S-BOX × 02”, changes in various parameters including PI control, and production control in accordance with the purpose can be performed.

Dedicated single use bottle

This product features a 500 mL single-use bottle as a standard, making it perfect for upscaling and commercial or industrial production. It is designed not only for safety, but with a focus on usability for more efficient culturing.

single use bottle 500ml

Various materials used in impellers, bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times even in small lots. There is no need to have a lot of Inventory due to the supply risk caused by overseas products.

Sterilized standard bottles

The bottles have already undergone EOG sterilization.
We have prepared certification for each lot.
An elution certificate can be provided (at an additional charge) upon request for production specifications.

Options

In principle, single-use bottles are provided only with a tube. Installation of the pH sensor and DO sensor will be performed in the clean bench. Contact separately if you want a non-contact sensor. We offer several optional products such as the continuous culture medium supply system, supply culture medium 37℃ adjustment system, bags provided as accessories, mechanism for humidity adjustment of gas flowing inside the cell culture tank, etc. We can also comply with special specifications. Please consult us for more details.

Dedicated data logger / software

Dedicated data logger / software

Using a dedicated data logger connected to a computer via Ethernet cable and our proprietary software, you can set an interval at which to acquire data on changes over time such as DO, pH, temperature, O2 flow rate, frequency, and number of revolutions during culturing, which can then be saved in CSV format.


Item Specifications
Name HiD4×4
Model HiD4-4
Temperature control method Hot plate + chiller /heating cooling type (PID control) with anti-excessive temperature rise function
Power transmission Linear shaft drive non-sealing type
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3 [℃] (37 [℃])
Max. up/down motion amplitude 40 [mm]
Max. impeller speed 150 [mm/s]
Function Temperature setting Digital setting, data output 1 - 5 [V]
Up/down motion setting Touch panel input, data output 1 - 10 [V]
Configuration Hot plate 235 [W]×4
Chiller Cooling type, 450 [W], HFC R-404A
Mixing motor Max. power 800 [W]
Mixing impellers [Standard] VM200
Culture tank Dimensions I.D. 94/87 x Depth 110 [mm] / approx. 300 [g]
Total capacity 0.5 [L]
Environment temp. range 10~35[℃]
Outer dimensions Main unit (HiD4×4) W680×H914×D480 [mm] Chiller (SCA-32) W205×H545×D405 [mm]
Weight Main unit (HiD4×4) approx. 70[kg] Chiller (SCA-32) approx. 28[kg]
Power supply AC100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×02
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
Control output pH (Hydrogen ion concentration) CO2ON/OFF control
DO (Dissolved oxygen) O2ON/OFF control
Data output pH (Hydrogen ion concentration) With data logger DC 0 - 5 [V]
*Mixing frequency and load factor data (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
Temperature With data logger DC 1 - 5 [V]
Casing SUS304 #300 Buffing, indoor type, non-drip proof, non-explosion proof specification
Installation Indoor stand-alone type
Outer dimensions/weight W600×H914×D500 [mm] *Does not include protrusions Approx. 70 [kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AV100 [V] 50/60[Hz] electrical outlet ×2 (for controller main unit, notebook PC)
N2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
O2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

Item Specifications
Data gathering
module
GM10
Material Polycarbonate
Outer dimensions W45.1×H111×D107.1[mm]
Weight approx. 0.25[kg]
Power supply From GM90PS power supply module
Power consumption 2.8 [W] or less
Power supply
module
GM90PS
Material Polycarbonate
Rated voltage AC100-240[V]
Outer dimensions W88×H135×D126.7[mm]
Weight approx. 0.55[kg]
module
base
GM90MB
Material Polycarbonate
Outer dimensions W57.7×H135×D103.5[mm]
Weight approx. 0.15[kg]