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HiD 4 × 4 Reactor

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Development of exclusive mixers・
and impellers by purpose of use

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Bioreactors

HiD 4×4

3D floating iPS cell differentiation induction BioReactorr

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

Patent No. 4316251, Other patents have either being applied for or already registered

The HiD 4 × 4 Reactor is the world’s first “Single-use mixer for 3D floating cell cultures” used exclusively for iPS cell differentiation induction. A BioReactor that achieves a high-volume production of uniform and homogeneous iPS cells is indispensable not only in regenerative medicine, but also in the “Use of human iPS cells in drug development” that aims at industrialization and commercial production. By promoting joint research and development with excellent research institutes or companies in Japan, we have succeeded in commercialization an iPS cell differentiation induction BioReactor for the first time in the world. 4, 8, 12, and 16 (or even more) such BioReactors can be controlled in a consolidated manner, and these are also best suited for screening. Moreover, we have also taken into consideration the adaptation to ES cells while performing development. With the help of the dedicated controller “S-BOX × 02”, changes in various parameters including PI control, and production control in accordance with the purpose can be performed.

single use　bottle　200ml

Various materials used in impellers, bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times even in small lots. There is no need to have a lot of Inventory due to the supply risk caused by overseas products.

Sterilized standard bottles

The bottles have already undergone EOG sterilization.
We have prepared certification for each lot.

Options

In principle, single-use bottles are provided only with a tube. Installation of the pH sensor and DO sensor will be performed in the clean bench. Contact separately if you want a non-contact sensor. We offer several optional products such as the continuous culture medium supply system, supply culture medium 37℃ adjustment system, bags provided as accessories, mechanism for humidity adjustment of gas flowing inside the cell culture tank, etc. We can also comply with special specifications. Please consult us for more details.

■HiD4×4 Specifications
Item		Specifications
Product name		HiD 4×4
Model		HiD 4-4
Temperature control type		Hot plate + Chiller overheating cooling method With excessive temperature rise prevention function
Linear-motion transmission method		Linear shaft drive non-sealing type
Performance	Temperature regulation range	Room temperature ＋5～20℃(normally set to 37℃)
	Temperature regulation accuracy	±0.3℃（37℃）
	Max. up/down motion amplitude	40mm
	Max. impeller speed	500mm/s
Function	Temperature setting	Digital setting
Function	Up/down motion setting	Touch panel input
Configuration	Hot plate	235Ｗ × 4
	Mixing motor	Max. output　800W
	Mixing impellers	Standard　VM200
Standard	Culture tank dimensions	Inner diameter 86(94) × 107 Depth [mm]
Standard	Culture tank total capacity	0.7Ｌ
Operating ambient temperature range		10～35℃
Outer dimensions	Reactor body （HiD4×4）	680W × 480D× 914H [mm]
Outer dimensions	Cooling chiller （SCA-32）	205W × 405D× 545H [mm]
Weight	Reactor body （HiD4×4）	Approx. 70kg
Weight	Cooling chiller （SCA-32）	Approx. 28kg
Rated power supply		AC-100V 50/60Hz

* The performance is the value at a room temperature of 20℃, rated power supply voltage 100 VAC 50 Hz, during no load.

■S-BOX×02 Specifications
Item	Specifications
Name	4-Unit 200 ml culture controller
Model	S-BOX×02
Display range / accuracy	pH (Hydrogen ion concentration)	0.00～14.00 [pH]
Display range / accuracy	DO (Dissolved oxygen)	0.0～130.0[%]
Control setting range	pH (Hydrogen ion concentration)	0.00～14.00 [pH]
Control setting range	DO (Dissolved oxygen)	0.0～130.0[%]
Control output	pH (Hydrogen ion concentration)	On/Off control by addition of CO₂ gas
Control output	DO (Dissolved oxygen)	On/Off control by addition of CO₂ gas
Recording medium	Data logger 20 ch
Power supply	AC100～230V　 50/60Hz
Case material	SUS304　#300 buffing Indoor type, non-drip proof, non-explosion proof specification
Installation method	Indoor stand-alone type
Outer dimensions	W600×D500×H914 [mm]　*Does not include projections
Ambient environment conditions	Temperature	Room temperature
Ambient environment conditions	Humidity	20～90%（No dew formation）
Utility	Power supply	100 VAC electrical outlet × 2 (For controller main unit, notebook PC)
	O₂	Flow rate 1 l/min or less Supply pressure 0.2 MPa　　 Connection port Φ6 tube one-touch joint
	CO₂	Flow rate 1 l/min or less Supply pressure 0.2 MPa　　 Connection port Φ6 tube one-touch joint
	AIR	Flow rate 1 l/min or less Supply pressure 0.2 MPa　 Connection port Φ6 tube one-touch joint
	* O₂, CO₂, and AIR must all be dry gases that do not contain corrosive components, dust, and oil mist