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HiD4×4 Reactor

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and impellers by purpose of use

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HSF Reactor
MRF Reactor
MRF-RB Reactor
VMF Reactor
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Reactor/TCS
SingleUse
BioReactor
HiD4x4 Reactor
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HiD 4×4

3D floating iPS cell differentiation induction BioReactorr

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

Patent No. 4316251, Other patents have either being applied for or already registered

The HiD 4 × 4 Reactor is the world’s first “Single-use mixer for 3D floating cell cultures” used exclusively for iPS cell differentiation induction. A BioReactor that achieves a high-volume production of uniform and homogeneous iPS cells is indispensable not only in regenerative medicine, but also in the “Use of human iPS cells in drug development” that aims at industrialization and commercial production. By promoting joint research and development with excellent research institutes or companies in Japan, we have succeeded in commercialization an iPS cell differentiation induction BioReactor for the first time in the world. 4, 8, 12, and 16 (or even more) such BioReactors can be controlled in a consolidated manner, and these are also best suited for screening. Moreover, we have also taken into consideration the adaptation to ES cells while performing development. With the help of the dedicated controller “S-BOX × 02”, changes in various parameters including PI control, and production control in accordance with the purpose can be performed.

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Dedicated single use bottle

This product features a 500 mL single-use bottle as a standard, making it perfect for upscaling and commercial or industrial production. It is designed not only for safety, but with a focus on usability for more efficient culturing.

Various materials used in impellers, bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times even in small lots. There is no need to have a lot of Inventory due to the supply risk caused by overseas products.

Sterilized standard bottles

The bottles have already undergone EOG sterilization.
We have prepared certification for each lot.
An elution certificate can be provided (at an additional charge) upon request for production specifications.

Options

In principle, single-use bottles are provided only with a tube. Installation of the pH sensor and DO sensor will be performed in the clean bench. Contact separately if you want a non-contact sensor. We offer several optional products such as the continuous culture medium supply system, supply culture medium 37℃ adjustment system, bags provided as accessories, mechanism for humidity adjustment of gas flowing inside the cell culture tank, etc. We can also comply with special specifications. Please consult us for more details.

Dedicated data logger / software

Using a dedicated data logger connected to a computer via Ethernet cable and our proprietary software, you can set an interval at which to acquire data on changes over time such as DO, pH, temperature, O2 flow rate, frequency, and number of revolutions during culturing, which can then be saved in CSV format.

▼　HiD4×4 Specifications（Click to open details）

Item		Specifications
Name		HiD4×4
Model		HiD4-4
Temperature control method		Hot plate + chiller /heating cooling type (PID control) with anti-excessive temperature rise function
Power transmission		Linear shaft drive non-sealing type
Performance	Temp. control range	Room temperature + 5 ～ 20℃ (normally set to 37℃)
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. up/down motion amplitude	40 [mm]
	Max. impeller speed	150 [mm/s]
Function	Temperature setting	Digital setting, data output 1 - 5 [V]
Function	Up/down motion setting	Touch panel input, data output 1 - 10 [V]
Configuration	Hot plate	235 [W]×4
	Chiller	Cooling type, 450 [W], HFC R-404A
	Mixing motor	Max. power 800 [W]
	Mixing impellers	[Standard] VM200
Culture tank	Dimensions	I.D. 94/87 x Depth 110 [mm] / approx. 300 [g]
Culture tank	Total capacity	0.5 [L]
Environment temp. range		10～35[℃]
Outer dimensions		Main unit (HiD4×4)	W680×H914×D480 [mm]	Chiller (SCA-32)	W205×H545×D405 [mm]
Weight		Main unit (HiD4×4)	approx. 70[kg]	Chiller (SCA-32)	approx. 28[kg]
Power supply		AC100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

▼　S-BOX×02 Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×02
Display range/accuracy	pH (Hydrogen ion concentration)		0.00～14.00 [pH]		Display accuracy: F.S±0.5 [%]
Display range/accuracy	DO (Dissolved oxygen)		0.00～20.00 [mg/L]		Display accuracy: F.S±0.5 [%]
Setting range	pH (Hydrogen ion concentration)		0.00～14.00 [pH]
Setting range	DO (Dissolved oxygen)		0.00～20.00 [mg/L]
Control output	pH (Hydrogen ion concentration)		CO₂ON/OFF control
Control output	DO (Dissolved oxygen)		O₂ON/OFF control
Data output	pH (Hydrogen ion concentration)		With data logger DC 0 - 5 [V] *Mixing frequency and load factor data (optional)		Accuracy: F.S±0.5 [%]
	DO (Dissolved oxygen)
	Temperature		With data logger DC 1 - 5 [V]
Casing	SUS304 #300 Buffing, indoor type, non-drip proof, non-explosion proof specification
Installation	Indoor stand-alone type
Outer dimensions/weight	W600×H914×D500 [mm] *Does not include protrusions Approx. 70 [kg]
Environment conditions	Temperature	5～45 [℃]		Humidity	20 - 85% RH (with no condensate)
Sensors	Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Sensors	Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities	Power supply	AV100 [V] 50/60[Hz] electrical outlet ×2 (for controller main unit, notebook PC)
	N₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
	O₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 tube one-touch joint
	*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

▼　Data logger Specifications（Click to open details）

Item		Specifications
Data gathering module GM10	Material	Polycarbonate
	Outer dimensions	W45.1×H111×D107.1[mm]
	Weight	approx. 0.25[kg]
	Power supply	From GM90PS power supply module
	Power consumption	2.8 [W] or less
Power supply module GM90PS	Material	Polycarbonate
	Rated voltage	AC100-240[V]
	Outer dimensions	W88×H135×D126.7[mm]
	Weight	approx. 0.55[kg]
module base GM90MB	Material	Polycarbonate
	Outer dimensions	W57.7×H135×D103.5[mm]
	Weight	approx. 0.15[kg]