Products & Services

Products & Services

Bioreactors

MRF-RB Reactor

Low-shear, high-dispersion mixing system combined with the RB Mixing System

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

MRF-RB Reactor
The MRF-RB Reactor is a BioReactor developed as a high-performance mixers with equipped with the low-shear, high-efficiency Satake Super-mix® RB Mixing System that has been used in up to 3,000-ton class biomass reactors. It is a next-generation mixing system that utilizes the boundary layer effect and principles of tornadoes, offering uniform dispersion performance with superb low shearing and low power performance. In continuous culture applications with the basic condition that there is no liquid surface fluctuation, this product is optimal not only for algae cultures, but also animal cell cultures and regenerative medicine. It is extremely easy to scale up, making it optimal for production consideration during the research stage for the actual machine. It is also possible to customize for single use.

Low-power, low shear action continuous BioReactor equipped with high-efficiency mixing system

RB Mixing system

In general, mixing is accomplished by using impellers to fluidize liquid. The role of impellers in the RB mixing system is not active mixing. The hint lies in natural flow and rectification action, and tornadoes, which are powerful enough to lift up houses. We wondered what the rectification action and tornado forces would create inside the mixing tank. The RB mixing system consists of an impeller, which generates a swirling flow inside the mixing tank, and radial blades that efficiently exchanges the swirling flow (boundary layer effect) toward the center at the bottom of the mixing tank with a tornado-shaped upward flow, forming a system that creates flow patterns that were unthinkable in conventional mixing. The ”RB” in the name comes from its Radial Blade component. Because the swirling flow is the main flow, the relative velocity difference near the blade is diminished, creating an extremely low shear action and superb uniform cell dispersion inside the culture tank, resulting in efficient mixing for biochemicals, pharmaceuticals, energy generation (water treatment), and other applications.

Mixing method comparison table

※Vtip:Blade tip peripheral speed

Mixing method Maximum upward flow velocity coefficient %
(vs. Vtip* ratio)
Impeller/liquid relative velocity difference coefficient
(shear velocity ratio)
Maximum possible mixing vertical ratio
(depth H/tank diameter D)
SUPER-MIX
RB mixing system
87 0.4 5 or more possible
Mixing with four baffle plates 30 0.8 Up to about 2
Mixing without baffle plates 15 0.4 Up to about 1.5
Mixing method comparison

Conventional
mixing

RB
Mixing System

CFD simulation analysis results

CFD simulation analysis results

High depth,
high circulation formation

Flow action (upper)
/shear stress (lower)


Item Specifications
Name MRF-RB Reactor
Model MRF-RB3000 MRF-RB10000
Temperature control method Band heater (PID control) with anti-excessive temperature rise function (Max. 80 [℃])
Power transmission Magnet drive non-sealing type
Gas supply device Shirasu porous glass (SPG) membrane type / sintered metal type
Performance Temp. control range Room temperature + 5 ~ 20℃ (normally set to 37℃)
Temp. accuracy ±0.3[℃](37[℃])
Rotation speed range 5~200[min-1]
Function Temp. setting Digital setting, data output 1 - 5 [V]
Speed setting Touch panel input, data output 0 - 10 [V]
Configuration Band heater 160 [W] 480 [W]
Mixer motor Max. output 100 [W]
Mixing impellers [Standard] RB Mixing System
Culture tank Dimensions I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]
Total capacity 3 [L] 10 [L]
Environment temp. range 10~35[℃]
Outer dimensions W360×H905×D485 [mm]
Weight approx. 30[kg] approx. 34[kg]
Power supply AC 100[V]、50/60[Hz]
*The performance is the value under room temperature 20℃, power supply AC 100 [V], 50 [Hz] and no load condition.

Item Specifications
Name Culture controller
Model S-BOX×10αII
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
FL (O2flow rate) 0.00~20.00 [mL/min]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) O2addition/subtraction step control
O2and N2ON/OFF control
O2PI control (slope set method: time, %)
FL (O2flow rate) PI control (slope set method: time, %)
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
*Load factor can be put out. (optional)
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
FL (O2flow rate)
Mixing frequency
Temperature With data logger
DC 1 - 5 [V]
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W350×H530×D400 [mm] ・ approx. 15[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessories: Mettler Toledo company Polarographic DO sensor/pH sensor
Option: Automatic System Research Co., Ltd. Optical DO sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
N2 Flow rate 50 [mL/min] or lower Supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.

Item Specifications
Name Culture controller
Model S-BOX×10 Simple
Display range/accuracy pH (Hydrogen ion concentration) 0.00~14.00 [pH] Display accuracy: F.S±0.5 [%]
DO (Dissolved oxygen) 0.00~20.00 [mg/L]
Setting range pH (Hydrogen ion concentration) 0.00~14.00 [pH]
DO (Dissolved oxygen) 0.00~10.00 [mg/L]
Control output pH (Hydrogen ion concentration) ON/OFF control
DO (Dissolved oxygen) ON/OFF control
Data output pH (Hydrogen ion concentration) With data logger
DC 0 - 5 [V]
Accuracy: F.S±0.5 [%]
DO (Dissolved oxygen)
Casing SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification
Installation Indoor tabletop type
Outer dimensions/weight W260×H350×D300 [mm] ・ approx. 12[kg]
Environment conditions Temperature 5~45 [℃] Humidity 20 - 85% RH (with no condensate)
Sensors Standard accessory: Automatic System Research Co., Ltd. Optical DO sensor
+ Mettler Toledo company pH sensor
Utilities Power supply AC100 [V] 50/60[Hz] electrical outlet × 2 (for controller unit and recorder)
O2 Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
CO2 Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
AIR Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa]
Connection port φ6 tube one-touch joint
*All of O₂, CO₂, and AIR must be dry gases that do not contain corrosive components, dust, and oil mist.