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Single-use BioReactor

VMF-50L/200L SUB

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The lineup consists of models from 50 to 200 L (development planned for up to 1,000 L) with tabletop models from 0.5 L to 10 L, which are self-standing from 50 L and up. This is the largest model in the standard VMF reactor series and a commercial production device that is perfectly scalable, offering an unprecedented starting size of 0.5 L. The S-BOX × 200 controller is included as a standard, offering full control over DO, pH, temperature, in-bag pressure, etc., and is equipped with four embedded pumps in a series, a dedicated internal digital data logger, Satake data logger software, and a BioReactor data collection system, etc. It also supports computerized system validation, and documents are provided for qualification confirmation during the design stage, during installation, and during operation. We also provide support for clients who are not accustomed to creating user requested specifications. We guarantee just-in-time supply in small lots of locally-manufactured single-use bags for sterilization validation. We can meet various demands required for commercial production, so please feel free to contact us.

Bag for single-use

Japan Patent No.6313635

●A completely hermetically sealed construction
　ensures a contamination-free environment.
●Since this is a 100% local product, we can
　always provide a stable supply even in small lots.
●Since sterilization has already been performed,
　you need not perform washing and sterilization
　at the time of use.
●The bag, impeller, shaft, and tube, etc. use
　materials conforming to USP Class VI
　(United States Pharmacopoeia Class 6).
●The DO/pH sensor is attached after high-pressure
　steam sterilizer in an autoclave.
●An optional single-use pressure sensor is also
　available.

Each sensor

Each sensor is attached to the culture tank as shown in the photo. The DO and pH sensors are attached using a Thermo Fisher Scientific bioreactor probe assembly with a sterile AseptiQuik Connector from CPC. The temperature sensor is installed inside a silicone tube sheath. It enables accurate temperature readings of production equipment without injecting water into the sheath.

CFD simulation analysis results

Model

Flow velocity
distribution

Turbulent energy

Shear stress

Consideration of commercial production scale-up
and utilization of numerical fluid calculation

▼　VMF-50L/200L SUB Specifications（Click to open details）

Item		Specifications
Name		VMF Reactor
Model		VMF-50L SUB^*1
Temp. control device		Rubber heater (PID control) with over-temperature protection function
Power transmission		Linear shaft drive non-sealing type
Gas supply type		Shirasu porous glass (SPG) membrane type / sintered metal type (selectable)
Performance^*2	Temp. control range	Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. shaft stroke	100 [mm]
	Max. shaft speed	800 [mm/s]
Function	Temp. setting	S-BOX×200 Touch panel input
Function	Vertical motion setting	S-BOX×200 Touch panel input
Configuration	Power of rubber heater	1.5 [kW]
	Power of motor	Max. output 800 [W]
	Mixing impellers	VM100+VM200
Culture tank	Dimensions	I.D. 369 x Depth 650 [mm]
Culture tank	Culture operation volume	40 - 45 [L]
Usage condition		10 - 35 [℃]
Outer dimensions		W780×D800×H2000 [mm]
Weight		Main unit approx. 135 [kg]
Power supply		Power is supplied from controller S-BOX × 200
*1　For VMF-200L SUB, please contact us for further information.
*2　The performance is the value under room temperature 20 [℃], power supply AC200 [V], 50 [Hz] and no load condition.

▼　S-BOX×200 Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX × 200
Control	pH, DO (Dissolved oxygen), FL (O₂ flow rate), TH (Temperature), Shaft motion, Medium weight, Pump ON/OFF
Display range/accuracy	TH (Temperature)	0.0 - 150.0 [℃]	DO (Dissolved oxygen)	0.0 - 100.0 [％]
	Linear shaft (mixing)	Stroke 0-100 [mm], Speed setting 0-800 [mm/s]	FL (O₂ flow rate)	0.00 - 5.00 [L/min]
	pH (Hydrogen ion concentration)	0.00 - 14.00 [－]	Electronic balance (for culture medium supply)	0.1 [g] - 21 [kg]
Setting range	TH (Temperature)	0.0 - 60.0 [℃]	DO (Dissolved oxygen)	0.0 - 100.0 [％]
	Linear shaft (mixing)	Stroke 0-100 [mm], Speed setting 0-800 [mm/s]	FL (O₂flow rate)	0.50 - 5.00 [L/min]
	pH (Hydrogen ion concentration)	0.00 - 14.00 [－]	Electronic balance (for culture medium supply)	0.1 [g] - 21 [ kg]
Control type	TH (Temperature)	PI control (slope set method: TIME, %)
	Linear shaft (mixing)	In-place control by shaft driver
	pH (Hydrogen ion concentration)	ON/OFF control of CO₂gas supply and alkaline water pump (selectable)
	DO (Dissolved oxygen)	PI control (slope set method: TIME, %) Using mass flow controller
	FL (O₂flow rate)	PI control (slope set method: TIME, %)
	FEED	ON/OFF control with FEED pump
	Culture medium supply/discharge	Supply and discharge by electronic balances, culture medium supply, and discharge pumps
Data output	pH (hydrogen ion concentration), DO (dissolved oxygen), FL (O₂flow rate), temperature, (option: mixing frequency ,Load factor by data logger 10ch)
MTA of outer surface	SUS304 (no coating) , indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor stand-alone type
Outer dimensions/weight	Outer dimensions W550×D550×H1000 [mm] *Does not include protrusions approx. 90 [kg]
Usage conditions	Temperature	5 - 45 [℃]	Humidity	20 - 85 [%] RH (No condensation)
Sensors	Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company


	(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)


Utilities	Power supply	1 [φ], AC200 [V], 30 [A] (2P + E, electrical outlet for 30 A hook-type plug) 1 gang
	O₂	Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	CO₂
	AIR
	*All of O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

Single-use System Up

Single-use Bottles／Single-use Bags

In biomedical products, regenerative medicine, and other such areas, single-use products are preferable from the perspectives of increased productivity and prevention of contamination. At SATAKE, we provide single-use products with total capacities ranging from 0.5 L to 200L (with plans to develop products of up to 1000L in capacity) to meet a wide range of needs. Our products achieve cell culture in a scalable manner from the laboratory to actual production.

＊　The 0.5 L single-use bottle is compatible with the "VMF Reactor VMF-05 and HiD".
＊　The 3L single-use bottle is compatible with the" VMF Reactor VMF-3" and also with 2 to 8 optional devices connected.

＊　The single-use bag is compatible with the "VMF Reactor VMF-10 and VMF Reactor VMF-50L / 200L SUB".

Various materials used in impellers,
bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times.

Proposal of application development
and customization

The single-use bag product lineup has a maximum capacity of 200 L, but we can also support bags of 500 to 1000 L capacity according to the application.
If you have a request for customized products that match the equipment, please contact us.

Sterilized standard bottles and bags

We prepare hard bottles and bags in the range of 0.5 L to 200L. Since EOG sterilization for hard bottles, γ sterilization for back has already been performed, you can start using these products as soon as you receive them. We have prepared certification for each lot.
Various testing has been conducted and conformity confirmed on each material used so you can use the products immediately without worry.

▼　Single-use Bottle/Bag Specifications（Click to open details）

Item	Specifications
Name	Single-use Bottles		Single-use Bags (3D)			Single-use Bags (2D)
Model	VMH-500	VMH-1000/3000	VMB-10	VMB-50	VMB-200	SCB-10	SCB-50
Culture operation volume	0.25 - 0.3 [L]	0.8 - 1.2/1.8 - 2.5 [L]	8 - 9 [L]	40 - 45 [L]	160 - 180 [L]	10 [L]	50 [L]
Dimensions	I.D. 94 (87) [mm]	I.D. 159 (138) [mm]	I.D. 206 [mm]	I.D. 369 [mm]	I.D. 590 [mm]	560×330 [mm]	740×705 [mm]
Dimensions	Depth 110 [mm]	Depth 202 [mm]	Depth 360 [mm]	Depth 650 [mm]	Depth 1015 [mm]	(Outer dimensions)	(Outer dimensions)
Ports^*1	Gas inlet/outlet, sampling, temperature sensor, DO sensor, pH sensor, culture medium replacement, inoculation port		Culture medium inlet/outlet, sampling, substrate inlet, cell inlet, air inlet/outlet, O₂inlet, temperature sensor, DO sensor, pH sensor			Gas inlet/outlet, harvest, sampling, cell inlet, culture medium inlet, spare^*2
Ports^*1
*1 The port equipped with the bag can be customized by consultation.
*2 Spare port only on SCB-50

Multipurpose Bag

This bag can be used for various purposes such as supply, discharge, and reagent use during continuous culture medium replacement. It can also be customized, so please feel free to inquire. γ-ray sterilization is employed to make this bag suitable for use with pharmaceuticals.

Bottles/Bags with Single-use Sensors

Single-use sensors (DO, pH) made by Mettler Toledo - well-known for culture-related products - are pre-attached to the bottle/bag. These can be used without autoclave sterilization, enabling preparation time to be shortened. They are also perfect for contamination prevention.