HiD 4×4 | Bioreactors | SATAKE MultiMix

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Bioreactors

Realization of Industrialization / Commercial Production / Scalability

Microbial culture
HSF Reactor

Animal cell culture
MRF Reactor

Animal cell culture
MRF-RB Reactor

Animal cell culture
VMF Reactor

Animal cell culture
VerSus Reactor

Regenerative medicine
VMF-WSUB Reactor/TCS

Single-use BioReactor

iPS cell culture
HiD4×4

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Development of exclusive mixers・
and impellers by purpose of use

Contracted numerical calculation

Evaluation of contracted cell culture

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Bioreactors

HSF Reactor
MRF Reactor
MRF-RB Reactor
VMF Reactor
VerSus Reactor
VMF-WSUB
Reactor/TCS
SingleUse
BioReactor
HiD4x4
Fittings/Options

HiD 4×4

3D floating iPS cell differentiation induction BioReactorr

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

The HiD 4 × 4 is the world’s first “Single-use mixer for 3D floating cell cultures” used exclusively for iPS cell differentiation induction. A BioReactor that achieves a high-volume production of uniform and homogeneous iPS cells is indispensable not only in regenerative medicine, but also in the “Use of human iPS cells in drug development” that aims at industrialization and commercial production. By promoting joint research and development with excellent research institutes or companies in Japan, we have succeeded in commercialization an iPS cell differentiation induction BioReactor for the first time in the world. 4, 8, 12, and 16 (or even more) such BioReactors can be controlled in a consolidated manner, and these are also best suited for screening. Moreover, we have also taken into consideration the adaptation to ES cells while performing development. With the help of the dedicated controller “S-BOX × 02”, changes in various parameters including PI control, and production control in accordance with the purpose can be performed.

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Single use bottle

This product features a 0.5 L single-use bottle as a standard, making it perfect for upscaling and commercial or industrial production. It is designed not only for safety, but with a focus on usability for more efficient culturing.

Various materials used in impellers, bottles, and bags

Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times even in small lots. There is no need to have a lot of Inventory due to the supply risk caused by overseas products.

Sterilized standard bottles

The bottles have already undergone EOG sterilization.
We have prepared certification for each lot.
An elution certificate can be provided (at an additional charge) upon request for production specifications.

Options

In principle, single-use bottles are provided only with a tube. Installation of the pH sensor and DO sensor will be performed in the clean bench. Contact separately if you want a non-contact sensor. We offer several optional products such as the continuous culture medium supply system, supply culture medium 37℃ adjustment system, bags provided as accessories, mechanism for humidity adjustment of gas flowing inside the cell culture tank, etc. We can also comply with special specifications. Please consult us for more details.

Dedicated data logger / software

Using a dedicated data logger connected to a computer via Ethernet cable and our proprietary software, you can set an interval at which to acquire data on changes over time such as DO, pH, temperature, O2 flow rate, frequency, and number of revolutions during culturing, which can then be saved in CSV format.

▼　HiD4×4 Specifications（Click to open details）

Item		Specifications
Name		HiD4×4
Model		HiD4-4
Temp. control device		Hot plate + chiller / heating & cooling type (PID control) with over-temperature protection function
Power transmission		Linear shaft drive non-sealing type
Performance^*1	Temp. control range	Room temperature + 5 - 20 [℃] (normally set to 37 [℃])
	Temp. accuracy	±0.3 [℃] (37 [℃])
	Max. shaft stroke	40 [mm]
	Max. shaft speed	150 [mm/s]
Function	Temp. setting	Touch panel input, data output DC1 - 5 [V]
Function	Vertical motion setting	Touch panel input, (option : mixing frequency data output DC0 - 5 [V] )
Configuration	Power of hot plate	235 [W]×4
	Chiller	Cooling type, 450 [W], HFC R-404A
	Power of motor	Max. output 800 [W]
	Mixing impeller	VM200
Culture tank	Dimensions	I.D. 94/87 x Depth 110 [mm]
Culture tank	Culture operation volume	0.25 - 0.3 [L]
Usage condition		10 - 35[℃]
Outer dimensions		Main unit (HiD4×4)	W680×D480×H914 [mm]	Chiller (SCA-32)	W205×D405×H545 [mm]
Weight		Main unit (HiD4×4)	Approx. 70 [kg]	Chiller (SCA-32)	Approx. 28 [kg]
Power supply		AC100 [V], 50/60 [Hz]
*1　The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

▼　S-BOX×02 Specifications（Click to open details）

Item	Specifications
Name	Culture controller
Model	S-BOX×02
Control	pH, DO (Dissolved oxygen)
Display range/accuracy	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]			Display accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
Setting range	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	pH (Hydrogen ion concentration)		0.00 - 14.00 [－]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
	DO (Dissolved oxygen)		0.00 - 20.00 [mg/L]
Control type	pH (Hydrogen ion concentration)		CO₂ON/OFF control
	pH (Hydrogen ion concentration)		CO₂ON/OFF control
	DO (Dissolved oxygen)		O₂ON/OFF control
	DO (Dissolved oxygen)		O₂ON/OFF control
Data output	pH (Hydrogen ion concentration)		With data logger DC0 - 5 [V] (option : Mixing frequencyand Load factor can be outputted.)			Accuracy: ±0.5 [%] F.S.
	pH (Hydrogen ion concentration)
	DO (Dissolved oxygen)
	DO (Dissolved oxygen)
	Temperature sensor		With data logger DC1 - 5 [V]
	Temperature sensor		With data logger DC1 - 5 [V]
MTA of outer surface	SUS304 (no coating) , indoor type, non-waterproof, non-explosion proof specification
Installation	Indoor stand-alone type
Outer dimensions/weight	W600×D500×H914 [mm] *Does not include protrusions approx. 70 [kg]
Usage conditions	Temperature	5 - 45 [℃]		Humidity	20 - 85 [%] RH (No condensation)
Sensors	Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company


	(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)


Utilities	Power supply	AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit, and laptop)
	N₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	O₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	CO₂	Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	AIR	Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
	*All of N₂, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

▼　Data logger Specifications（Click to open details）

Item		Specifications
Data gathering module GM10	Material	Polycarbonate
	Outer dimensions	W45.1×H111×D107.1[mm]
	Weight	approx. 0.25[kg]
	Power supply	From GM90PS power supply module
	Power consumption	2.8 [W] or less
Power supply module GM90PS	Material	Polycarbonate
	Rated voltage	AC100-240[V]
	Outer dimensions	W88×H135×D126.7[mm]
	Weight	approx. 0.55[kg]
module base GM90MB	Material	Polycarbonate
	Outer dimensions	W57.7×H135×D103.5[mm]
	Weight	approx. 0.15[kg]